1. 重组蛋白
  2. CAR-T related Proteins CD Antigens Biotinylated Proteins
  3. CD4 T Cell CD Proteins Dendritic Cell CD Proteins
  4. CD4 蛋白, Mouse (Biotinylated, HEK293, His-Avi)

CD4 蛋白, Mouse (Biotinylated, HEK293, His-Avi)

目录号: HY-P77517
产品使用指南

CD4 是一种在免疫反应中至关重要的糖蛋白,可与 IL-16 结合并形成同型二聚体。它增强辅助 T 细胞介导的免疫反应并调节防御革兰氏阴性细菌和钙信号传导等过程。CD4 蛋白, Mouse (Biotinylated, HEK293, His-Avi) 是重组的 CD4 蛋白,由 HEK293 表达,带有 C-His; C-Avi标签。

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SPR 检测服务   重组蛋白定制服务

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规格 是否有货
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描述

CD4 is a glycoprotein critical in immune responses that binds to interleukin-16 and forms a homodimer. It enhances helper T cell-mediated immune responses and regulates processes such as defense against Gram-negative bacteria and calcium signaling. CD4 Protein, Mouse (Biotinylated, HEK293, His-Avi) is the recombinant mouse-derived CD4 protein, expressed by HEK293 , with C-His, C-Avi labeled tag.

研究背景

CD4 是参与免疫反应的关键糖蛋白,预计具有多种功能,包括白细胞介素 16 结合活性、白细胞介素 16 受体活性和蛋白质同源二聚化活性。CD4 在辅助 T 细胞增强适应性免疫反应中发挥着关键作用,其作用于革兰氏阴性菌的防御反应、钙介导信号传导的正向调节以及肽基酪氨酸磷酸化的正向调节等过程的上游。CD4 位于内质网、质膜外侧和膜筏内,在不同组织中表现出偏向表达,特别是在胸腺中表达水平为 RPKM 167.0,在脾中表达水平为 RPKM 15.0,强调了其表达水平。免疫功能的重要性及其与免疫缺陷的潜在关系 79.

种属

Mouse

表达系统

HEK293

标签

C-His;C-Avi

蛋白编号

NP_038516.1 (K27-T394)

基因 ID
蛋白结构
N-term
CD4 (K27-T394)
Accession # NP_038516.1
His-Avi
C-term
同用名
T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD4
氨基酸序列

KTLVLGKEGESAELPCESSQKKITVFTWKFSDQRKILGQHGKGVLIRGGSPSQFDRFDSKKGAWEKGSFPLIINKLKMEDSQTYICELENRKEEVELWVFKVTFSPGTSLLQGQSLTLTLDSNSKVSNPLTECKHKKGKVVSGSKVLSMSNLRVQDSDFWNCTVTLDQKKNWFGMTLSVLGFQSTAITAYKSEGESAEFSFPLNFAEENGWGELMWKAEKDSFFQPWISFSIKNKEVSVQKSTKDLKLQLKETLPLTLKIPQVSLQFAGSGNLTLTLDKGTLHQEVNLVVMKVAQLNNTLTCEVMGPTSPKMRLTLKQENQEARVSEEQKVVQVVAPETGLWQCLLSEGDKVKMDSRIQVLSRGVNQT

分子量

Approximately 52.83 kDa.

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD4 Protein, Mouse (Biotinylated, HEK293, His-Avi)
目录号:
HY-P77517
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