CD4 蛋白, Mouse (HEK293, His)

CD4 is a glycoprotein critical in immune responses that binds to interleukin-16 and forms a homodimer. It enhances helper T cell-mediated immune responses and regulates processes such as defense against Gram-negative bacteria and calcium signaling. CD4 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD4 protein, expressed by HEK293 , with C-His, C-6*His labeled tag. The total length of CD4 Protein, Mouse (HEK293, His) is 368 a.a., with molecular weight of 43-60 kDa.

CD4 是参与免疫反应的关键糖蛋白，预计具有多种功能，包括白细胞介素 16 结合活性、白细胞介素 16 受体活性和蛋白质同源二聚化活性。CD4 在辅助 T 细胞增强适应性免疫反应中发挥着关键作用，其作用于革兰氏阴性菌的防御反应、钙介导信号传导的正向调节以及肽基酪氨酸磷酸化的正向调节等过程的上游。CD4 位于内质网、质膜外侧和膜筏内，在不同组织中表现出偏向表达，特别是在胸腺中表达水平为 RPKM 167.0，在脾中表达水平为 RPKM 15.0，强调了其表达水平。免疫功能的重要性及其与免疫缺陷的潜在关系 79.

Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells. The ED50 this effect is ≤3 μg/mL, corresponding to a specific activity is ≥355.333 units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells. The ED₅₀ this effect is 2.816 μg/mL, corresponding to a specific activity is 355.114 units/mg.

Mouse

HEK293

C-6*His

NP_038516.1 (K27-T394)

12504  [NCBI]

KTLVLGKEGESAELPCESSQKKITVFTWKFSDQRKILGQHGKGVLIRGGSPSQFDRFDSKKGAWEKGSFPLIINKLKMEDSQTYICELENRKEEVELWVFKVTFSPGTSLLQGQSLTLTLDSNSKVSNPLTECKHKKGKVVSGSKVLSMSNLRVQDSDFWNCTVTLDQKKNWFGMTLSVLGFQSTAITAYKSEGESAEFSFPLNFAEENGWGELMWKAEKDSFFQPWISFSIKNKEVSVQKSTKDLKLQLKETLPLTLKIPQVSLQFAGSGNLTLTLDKGTLHQEVNLVVMKVAQLNNTLTCEVMGPTSPKMRLTLKQENQEARVSEEQKVVQVVAPETGLWQCLLSEGDKVKMDSRIQVLSRGVNQT

43-60kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.