CD40L/CD154/TRAP 蛋白, Human (Biotinylated, HEK293, Fc-Avi)

CD40L/CD154/TRAP proteins stimulate T cell proliferation and cytokine production, acting as ligands for integrins and CD40 receptors to activate various cell-dependent effects such as B cell activation and NF-κ-B signaling. CD40L/CD154/TRAP Protein, Human (Biotinylated, HEK293, Fc-Avi) is the recombinant human-derived CD40L/CD154/TRAP protein, expressed by HEK293 , with N-hFc, N-Avi labeled tag. The total length of CD40L/CD154/TRAP Protein, Human (Biotinylated, HEK293, Fc-Avi) is 149 a.a., with molecular weight of 50-55 kDa.

CD40L，也称为 CD154 或 TRAP 蛋白，具有细胞因子的功能，并作为 CD40/TNFRSF5 的配体，协调一系列免疫反应。它在 T 细胞激活中发挥着关键作用，充当有效的共刺激剂，增强 T 细胞增殖和细胞因子的产生，特别是 IL4 和 IL10。在 T 细胞上交联后，CD40L 会产生与 TCR/CD3 连接和 CD28 共刺激协同的共刺激信号。此外，CD40L 还可诱导 NF-kappa-B 的激活，触发 T 细胞中激酶 MAPK8 和 PAK2 的激活，并促进 CD28 异构体 3 的酪氨酸磷酸化。除了 T 细胞调节之外，CD40L 在不存在的情况下还可介导 B 细胞增殖。共刺激并在 IL4 存在的情况下促进 IgE 产生，有助于免疫球蛋白类别转换。此外，它作为整合素的配体，特别是 ITGA5:ITGB1 和 ITGAV:ITGB3，并与 CD40 受体合作激活 CD40-CD40LG 信号传导，发挥细胞类型依赖性效应，例如 B 细胞激活、NF- kappa-B 信号传导和抗凋亡信号传导。

Immobilized Human CD40 His at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD40 Ligand Avi-Fc with a linear range of 1-31 ng/mL.

Human

HEK293

N-hFc;N-Avi

P29965 (M113-L261)

959  [NCBI]

50-55 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized a 0.22 μm filtered solution of Tris with Glycine, Arginine and NaCl, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.