CD45 蛋白, Human (Biotinylated, HEK293, His-Avi)

Receptor-type tyrosine-protein phosphatase C (PTPRC) is a receptor type PTP and an essential positive regulator of T- and B-cell antigen receptor signaling. PTPRC functions through direct interaction with antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. PTPRC also suppresses JAK kinases as a regulator of cytokine receptor signaling. CD45 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CD45 protein, expressed by HEK293 , with C-His, C-Avi labeled tag. The total length of CD45 Protein, Human (Biotinylated, HEK293, His-Avi) is 391 a.a., with molecular weight of 80-110 kDa.

受体型酪氨酸蛋白磷酸酶 C (PTPRC) 是蛋白酪氨酸磷酸酶 (PTP) 家族的一员，也被称作 CD45, 是一种跨膜糖蛋白。已知 PTPs 是调节多种细胞过程的信号分子，包括细胞生长、分化、有丝分裂和致癌转化。
PTPRC 包含一个胞外结构域、一个跨膜片段和两个串联胞质内催化结构域，因此被归类为受体型 PTP。
PTPRC 已被证明是 T 细胞和 B 细胞抗原受体信号传导的重要调控者，PTPRC 通过与 DPP4 结合，募集和去磷酸化 SKAP1 和 FYN，正向调节 T 细胞的共激活。PTPRC 也使 LYN 去磷酸化，从而调节 LYN 活性。
PTPRC 通过与抗原受体复合物组分的直接相互作用或通过激活抗原受体信号传导所需的各种 Src 家族激酶发挥作用。PTPRC 也抑制 JAK 激酶，因此作为细胞因子受体信号传导的调节因子。
PTPRC 基因有许多选择性剪接的转录物变体，它们编码不同的同种异构体^[1][2][3]。

Immobilized Anti-CD45 Antibody at 2 μg/mL (100μL/well) on the plate. Dose response curve for Biotinylated Human CD45, His Tag with the EC₅₀ of <0.12 μg/mL determined by ELISA.

Human

HEK293

C-His;C-Avi

P08575-4 (Q26-K416)

5788  [NCBI]

QSPTPSPTDAYLNASETTTLSPSGSAVISTTTIATTPSKPTCDEKYANITVDYLYNKETKLFTAKLNVNENVECGNNTCTNNEVHNLTECKNASVSISHNSCTAPDKTLILDVPPGVEKFQLHDCTQVEKADTTICLKWKNIETFTCDTQNITYRFQCGNMIFDNKEIKLENLEPEHEYKCDSEILYNNHKFTNASKIIKTDFGSPGEPQIIFCRSEAAHQGVITWNPPQRSFHNFTLCYIKETEKDCLNLDKNLIKYDLQNLKPYTKYVLSLHAYIIAKVQRNGSAAMCHFTTKSAPPSQVWNMTVSMTSDNSMHVKCRPPRDRNGPHERYHLEVEAGNTLVRNESHKNCDFRVKDLQYSTDYTFKAYFHNGDYPGEPFILHHSTSYNSK

80-110 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.