CD47 蛋白, Rat (HEK293, Fc)

The CD47 protein acts as a THBS1 receptor and regulates integrin signaling, promoting cell-cell interactions. It plays a role in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, immune regulation, and memory formation. CD47 Protein, Rat (HEK293, Fc) is the recombinant rat-derived CD47 protein, expressed by HEK293 , with C-hFc labeled tag.

CD47 蛋白是一种促进细胞间相互作用的粘附蛋白。它作为血小板反应蛋白 THBS1 的受体，并通过激活异三聚体 G 蛋白来调节整合素信号传导。CD47参与多种生物过程，包括信号转导、心血管稳态、炎症、细胞凋亡、血管生成、细胞自我更新和免疫调节。它还在调节肺内皮素 EDN1 信号和一氧化二氮 (NO) 信号中发挥作用，有助于血压调节。此外，CD47 对于海马体的记忆形成和突触可塑性也很重要。它作为 SIRPA 的受体，防止未成熟树突状细胞的成熟并抑制成熟树突状细胞产生细胞因子。与 SIRPG 的相互作用可增强细胞间粘附、T 细胞增殖和 T 细胞活化。CD47 正向调节 T 细胞中 FAS 依赖性细胞凋亡并抑制血管生成。它还可能参与正常衰老过程中的代谢失调，并调节伤口愈合和干细胞自我更新。CD47 可能在膜运输、整合素依赖性信号转导和防止红细胞过早消除中发挥作用。它与 THBS1、SIRPA、FAS/CD95、SIRPG、UBQLN1、UBQLN2 和纤维蛋白原相互作用。

1.Immobilized Mouse SIRP alpha, His Tag at 2 μg/mL (100μl/well) on the plate. Dose response curve for Rat CD47, hFc Tag with the EC₅₀ of 88.7 ng/mL determined by ELISA.
2.Measured by its binding ability in a functional ELISA. Immobilized Rat CD47 at 10 μg/mL (100 μL/well) can bind Biotinylated Mouse SIRP alpha. The ED₅₀ for this effect is 0.387 μg/mL.

Rat

HEK293

C-hFc

P97829-1 (Q19-K140)

29364  [NCBI]

QLLLSKVKSVEFTSCNDTVVIPCKVLNVEAQSTDEMFVKWKLNKSYIFIYDGNKNSTTREQNFTSAKISVSDLLKGIASLTMDTHEAVVGNYTCEVTELSREGKTVIELKNRPVSWFSTNEK

50-70 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.