1. 重组蛋白
  2. Immune Checkpoint Proteins CD Antigens
  3. Stimulatory Immune Checkpoint Molecules NK Cell CD Proteins Macrophage CD Proteins Stem Cell CD Proteins Platelet CD Proteins
  4. CD48
  5. SLAMF2/CD48 蛋白, Human (HEK293, His)

SLAMF2/CD48 蛋白, Human (HEK293, His)

目录号: HY-P72919
产品使用指南

SLAMF2/CD48 蛋白是一种 GPI 锚定糖蛋白,通过与 CD2 和 CD244 相互作用来调节免疫细胞功能。SLAMF2/CD48 蛋白, Human (HEK293, His) 是重组的 SLAMF2/CD48 蛋白,由 HEK293 表达,带有 C-His 标签。SLAMF2/CD48 蛋白, Human (HEK293, His) 全长 194 个氨基酸,分子量约为 ~40 kDa。

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描述

SLAMF2/CD48 protein is a GPI-anchored glycoprotein that regulates immune cell function by interacting with CD2 and CD244. SLAMF2/CD48 Protein, Human (HEK293, His) is the recombinant human-derived SLAMF2/CD48 protein, expressed by HEK293 , with C-His labeled tag. The total length of SLAMF2/CD48 Protein, Human (HEK293, His) is 194 a.a., with molecular weight of ~40 kDa.

研究背景

SLAMF2/CD48 蛋白是一种糖基磷脂酰肌醇 (GPI) 锚定的细胞表面糖蛋白,通过其 N 端免疫球蛋白结构域与细胞表面受体(如 2B4/CD244 或 CD2)相互作用,在免疫细胞调节和激活中发挥关键作用。在T细胞信号转导中,SLAMF2与CD2结合,促进Src家族蛋白激酶LCK和LAT有效招募至TCR/CD3复合物,从而促进LCK磷酸化和随后的激活。此外,SLAMF2 诱导 CD244 的细胞质免疫受体酪氨酸开关基序 (ITSM) 磷酸化,启动一系列信号传导事件,最终形成免疫突触,并通过 T 淋巴细胞和颗粒酶直接释放含有穿孔素和颗粒酶的溶细胞颗粒。NK 细胞。值得注意的是,SLAMF2 直接与 CD2、CD244 和 LCK 相互作用,突显其在免疫细胞功能和信号通路中的复杂参与。

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

P09326 (Q27-S220)

基因 ID

962  [NCBI]

蛋白结构
N-term
CD48 (Q27-S220)
Accession # P09326
His
C-term
同用名
CD48 antigen; SLAMF2; TCT.1; CD48; BCM1; BLAST1
氨基酸序列

MCSRGWDSCLALELLLLPLSLLVTSIQGHLVHMTVVSGSNVTLNISESLPENYKQLTWFYTFDQKIVEWDSRKSKYFESKFKGRVRLDPQSGALYISKVQKEDNSTYIMRVLKKTGNEQEWKIKLQVLDPVPKPVIKIEKIEDMDDNCYLKLSCVIPGESVNYTWYGDKRPFPKELQNSVLETTLMPHNYSRCYTCQVSNSVSSKNGTVCLSPPCTLARS

分子量

Approximately 40 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
SLAMF2/CD48 Protein, Human (HEK293, His)
目录号:
HY-P72919
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