1. 重组蛋白
  2. CD Antigens
  3. T Cell CD Proteins B Cell CD Proteins Dendritic Cell CD Proteins Cell Adhesion-related CD Proteins
  4. CD5
  5. CD5 蛋白, Rat (HEK293, His)

CD5 蛋白, Rat (HEK293, His)

目录号: HY-P74283
COA 产品使用指南

CD5 蛋白是一种关键的 T 细胞受体,通过与 CD72/LYB-2 相互作用来调节 T 细胞增殖。它与 PTPN6/SHP-1 的潜在相互作用强调了它参与关键信号通路。CD5 蛋白, Rat (HEK293, His) 是重组的 CD5 蛋白,由 HEK293 表达,带有 C-His 标签。CD5 蛋白, Rat (HEK293, His) 全长 345 个氨基酸,分子量约为 ~50-56 kDa。

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规格 价格 是否有货 数量
Free Sample   Apply now  
5 μg ¥470 In-stock
10 μg ¥800 In-stock
50 μg ¥2250 In-stock
100 μg ¥3800 In-stock
500 μg ¥10650 In-stock
1 mg ¥18000 In-stock
> 1 mg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

CD5 protein, a pivotal T-cell receptor, regulates T-cell proliferation through interactions with CD72/LYB-2. Its potential interaction with PTPN6/SHP-1 underscores its involvement in critical signaling pathways. CD5 Protein, Rat (HEK293, His) is the recombinant rat-derived CD5 protein, expressed by HEK293 , with C-His labeled tag. The total length of CD5 Protein, Rat (HEK293, His) is 345 a.a., with molecular weight of ~50-56 kDa.

研究背景

CD5 蛋白作为受体在 T 细胞增殖的调节中发挥着至关重要的作用。它与 CD72/LYB-2 相互作用,还与 PTPN6/SHP-1 潜在相互作用,突出了它参与重要的信号传导途径。

生物活性

Immobilized Rat CD5 Protein at 1 μg/mL (100 μL/well) can bind Anti-CD5 Antibody, the ED50 for this effect is 18.65 ng/mL.

  • Immobilized Rat CD5 Protein at 1 μg/mL (100 μL/well) can bind Anti-CD5 Antibody, the ED50 for this effect is 18.65 ng/mL.
种属

Rat

表达系统

HEK293

标签

C-His

蛋白编号

P51882 (Q24-P368)

基因 ID
蛋白结构
N-term
CD5 (Q24-P368)
Accession # P51882
His
C-term
同用名
T-cell surface glycoprotein CD5; Ly-1; Lyt-1; CD5; Leu-1
氨基酸序列

QSGRGFVGAQVMLSGSNSKCQGLVEVQMNGMKTVCSSSWRLSQDLWKNANEASTVCQQLGCGNPLALGHLTLWNRPKNQILCQGPPWSFSNCSTSSLGQCLPLSLVCLEPQKTTPLPTTTLPTTMPEPTAPPRLQLVPGHEGLRCTGVVEFYNGSRGGTILYKAKARPVDLGNLICKSLQCGSFLTHLSRIETAGTPAPAELRDPRPLPIRWEAQNGSCTSLQQCFQKTTVQEGSQALAVVCSDFQPKVQSRLVGGSSVCEGIAEVRQRSQWAALCDSSAARGPGRWEELCQEQQCGNLISFHVMDADRTSPGVLCTQEKLSQCYQLQKKTHCKRVFITCKDPNP

分子量

Approximately 50-56 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD5 Protein, Rat (HEK293, His)
目录号:
HY-P74283
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