CD55/DAF 蛋白, Human (HEK293, hFc)

The CD55/DAF protein is critical in the immune system, recognizing C4b and C3b fragments, interfering with their enzymatic conversion and preventing the formation of complement cascade amplifying convertase. This regulatory mechanism alleviates complement-induced damage by inhibiting complement activation. CD55/DAF Protein, Human (HEK293, hFc) is the recombinant human-derived CD55/DAF protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD55/DAF Protein, Human (HEK293, hFc) is 319 a.a., with molecular weight of 95-105 kDa.

CD55/DAF 蛋白通过识别 C4 和 C3 激活过程中产生的 C4b 和 C3b 片段，在免疫系统中发挥着至关重要的作用。它与细胞相关的 C4b 和 C3b 多肽的相互作用干扰它们催化 C2 和因子 B 转化为酶活性 C2a 和 Bb 的能力，从而阻止 C4b2a 和 C3bBb（补体级联的扩增转化酶）的形成。这种干扰作为一种调节机制，抑制补体激活并阻止 C3 和 C5 转化酶的形成，从而减轻补体诱导的损伤。值得注意的是，CD55/DAF 在微生物感染过程中还充当柯萨奇病毒 A21 以及柯萨奇病毒 B1、B3 和 B5 的受体，突出了其在免疫调节和病毒识别中的多种作用。

Measured by its ability to bind human CD97-His in a functional ELISA.

Human

HEK293

C-hFc

P08174 (D35-S353)

1604  [NCBI]

95-105 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.