CD5L 蛋白, Mouse (HEK293, His)

The CD5L protein is primarily expressed by macrophages and regulates inflammatory responses in infection or atherosclerosis. CD5L is incorporated into adipocytes via CD36-mediated endocytosis, thereby inhibiting lipid droplet size and affecting lipolysis. CD5L Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD5L protein, expressed by HEK293 , with C-6*His labeled tag.

CD5L 蛋白是脂质合成的关键调节因子，主要由淋巴组织和发炎组织中的巨噬细胞表达，在感染或动脉粥样硬化等情况下协调炎症反应。它的影响延伸到脂肪细胞，通过 CD36 介导的内吞作用并入成熟脂肪细胞后，它会抑制脂滴大小并影响脂肪分解。通过与胞质 FASN 结合，CD5L 抑制脂肪酸合酶活性，导致三酰甘油分解为甘油和游离脂肪酸。在肥胖的情况下，CD5L 诱导的脂肪分解通过将炎症巨噬细胞募集到脂肪组织中而导致炎症和胰岛素抵抗。此外，CD5L 对辅助性 T Th17 细胞中的代谢途径发挥调节控制作用，通过调节脂质含量和限制 RORC（Th17 细胞分化的主要转录因子）胆固醇配体的合成来影响促炎基因的表达。CD5L 尤其存在于非致病性 Th17 细胞中，它会改变多不饱和脂肪酰基含量，影响代谢蛋白，从而限制 RORC 活性。此外，CD5L 通过与 IgM 结合、干扰 Fcalpha/mu 受体结合以及促进产生高亲和力 IgG 自身抗体的长寿命浆细胞的发育，在肥胖相关自身免疫中发挥作用。除了其代谢功能外，CD5L 还充当巨噬细胞的凋亡抑制剂，促进巨噬细胞的存活，抵抗动脉粥样硬化中氧化脂质的凋亡作用。此外，它有助于对微生物感染的早期反应，充当模式识别受体并促进自噬。CD5L 的多方面作用强调了其在不同生理过程中的重要性及其在各种病理条件下的潜在影响。

Mouse

HEK293

C-6*His

Q9QWK4 (E22-V352)

11801  [NCBI]

ESPTKVQLVGGAHRCEGRVEVEHNGQWGTVCDDGWDRRDVAVVCRELNCGAVIQTPRGASYQPPASEQRVLIQGVDCNGTEDTLAQCELNYDVFDCSHEEDAGAQCENPDSDLLFIPEDVRLVDGPGHCQGRVEVLHQSQWSTVCKAGWNLQVSKVVCRQLGCGRALLTYGSCNKNTQGKGPIWMGKMSCSGQEANLRSCLLSRLENNCTHGEDTWMECEDPFELKLVGGDTPCSGRLEVLHKGSWGSVCDDNWGEKEDQVVCKQLGCGKSLHPSPKTRKIYGPGAGRIWLDDVNCSGKEQSLEFCRHRLWGYHDCTHKEDVEVICTDFDV

43-55 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.