CD6 蛋白, Human (HEK293, Fc)

The CD6 protein is a cell adhesion molecule that mediates cell-cell contacts and regulates T cell responses through interaction with ALCAM/CD166. It functions as a costimulatory molecule, promoting T cell activation, proliferation, and contributing to the formation of immune synapses. CD6 Protein, Human (HEK293, Fc) is the recombinant human-derived CD6 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD6 Protein, Human (HEK293, Fc) is 381 a.a., with molecular weight of ~67.3 kDa.

CD6 蛋白是一种关键的细胞粘附分子，通过与 ALCAM/CD166 相互作用，在介导细胞间接触和协调 T 细胞反应方面发挥着核心作用。这种相互作用对于调节 T 细胞活化和增殖至关重要，有助于免疫突触的形成和成熟。除了在 T 细胞反应中的作用外，CD6 还充当钙依赖性模式受体，结合并聚集革兰氏阳性菌和革兰氏阴性菌，并响应革兰氏菌脂多糖 (LPS)，下游激活信号级联和 MAP 激酶- 阴性细菌。CD6参与炎症反应包括在LPS刺激后介导促炎细胞因子的分泌。CD6 与多种蛋白质相互作用，包括 TCR/CD3 复合物亚基 CD3E、LCP2、VAV1 以及 LGALS1 和 LGALS3 的糖基化胞外结构域。值得注意的是，与 LGALS1 或 LGALS3 的相互作用会抑制 CD6 与 ALCAM 的结合，从而进一步深入了解 CD6 介导的细胞功能的复杂调节机制。

Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8×10⁴ cells/well are added to CD6-Fc coated plates (5 μg/mL and 100 μL/well) in the ppresence of 10 μg/ml PHA, approximately > 50% cells will adhere specifically after 60 minutes at 37°C.

Human

HEK293

C-hFc

P30203 (H18-E398)

923  [NCBI]

MWLFFGITGLLTAALSGHPSPAPPDQLNTSSAESELWEPGERLPVRLTNGSSSCSGTVEVRLEASWEPACGALWDSRAAEAVCRALGCGGAEAASQLAPPTPELPPPPAAGNTSVAANATLAGAPALLCSGAEWRLCEVVEHACRSDGRRARVTCAENRALRLVDGGGACAGRVEMLEHGEWGSVCDDTWDLEDAHVVCRQLGCGWAVQALPGLHFTPGRGPIHRDQVNCSGAEAYLWDCPGLPGQHYCGHKEDAGAVCSEHQSWRLTGGADRCEGQVEVHFRGVWNTVCDSEWYPSEAKVLCQSLGCGTAVERPKGLPHSLSGRMYYSCNGEELTLSNCSWRFNNSNLCSQSLAARVLCSASRSLHNLSTPEVPASVQTVTIESSVTVKIENKESRE

Approximately 67.3 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.