CD63 蛋白, Rat (HEK293, His)

The CD63 protein serves as a cell surface receptor for TIMP1 and is critical for activating signaling cascades. It contributes to ITGB1 and integrin signaling, activating AKT, FAK/PTK2 and MAP kinases to promote cell survival, cytoskeletal reorganization, adhesion, spreading and migration. CD63 Protein, Rat (HEK293, His) is the recombinant rat-derived CD63 protein, expressed by HEK293 , with N-His labeled tag. The total length of CD63 Protein, Rat (HEK293, His) is 101 a.a., with molecular weight of ~24 & 19 & 15 kDa, respectively.

CD63 蛋白作为 TIMP1 的细胞表面受体，在细胞信号级联的激活中发挥着至关重要的作用。它有助于激活 ITGB1 和整合素信号传导，从而激活 AKT、FAK/PTK2 和 MAP 激酶。它通过激活 AKT 和 FAK/PTK2 参与促进细胞存活、肌动蛋白细胞骨架重组、细胞粘附、扩散和迁移。CD63 还通过调节 KDR/VEGFR2 内化参与 VEGFA 信号转导。此外，它在细胞内囊泡运输过程中发挥作用，对于 PMEL 管腔结构域的正常运输至关重要，而 PMEL 管腔结构域对于黑素细胞的发育和成熟至关重要。CD63 通过调节 SELP 运输进一步参与白细胞与内皮细胞的粘附。虽然它可能参与响应 Ms4a2/FceRI 刺激的肥大细胞脱颗粒，但其在响应其他刺激的脱颗粒中的作用仍然有限。CD63 与 TIMP1 和 ITGB1 相互作用，将 TIMP1 募集到 ITGB1，与 CD9 和 ITGB3 形成复合物，并与 PMEL、KDR/VEGFR2 和 SYT7 相互作用。

Measured by its binding ability in a functional ELISA. When Recombinant Human BST-2 is immobilized at 2.5 μg/mL (100 μL/well), Recombinant CD63 binds with an ED₅₀ of 0.9159 μg/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Human BST-2 is immobilized at 2.5 μg/mL (100 μL/well), Recombinant CD63 binds with an ED₅₀ of 0.9159 μg/mL.

Rat

HEK293

N-His

P28648 (A103-V203)

29186  [NCBI]

AGYVFRDQVKSEFSKSFQKQMQNYLTDNKTATILDKLQKENKCCGASNYTDWERIPGMAKDRVPDSCCINITVGCGNDFKESTIHTQGCVETIAAWLRKNV

The protein migrates as an approximately 24&19&15 kDa band under reducing SDS-PAGE due to glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.