2. 重组蛋白
  3. CD Antigens
  4. Macrophage CD Proteins Monocyte CD Proteins Stem Cell CD Proteins Dendritic Cell CD Proteins Epithelial cell CD Proteins
  5. CD68
  6. CD68 蛋白, Human (sf9, His)

CD68 蛋白, Human (sf9, His)

目录号: HY-P74279
产品使用指南

CD68 蛋白对于组织巨噬细胞的吞噬活动至关重要，包括溶酶体代谢以及与细胞和病原体的相互作用。它与凝集素或选择素结合，帮助特定巨噬细胞亚群的靶向迁移。CD68从内体和溶酶体到质膜的快速再循环使得巨噬细胞能够爬行或与带有选择素的底物和其他细胞相互作用。CD68 蛋白, Human (sf9, His) 是重组的 CD68 蛋白，由 Sf9 insect cells 表达，带有 C-His 标签。CD68 蛋白, Human (sf9, His) 全长 319 个氨基酸，分子量约为 ~68 kDa。

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描述

CD68 protein is essential for phagocytic activities in tissue macrophages, including lysosomal metabolism and interactions with cells and pathogens. It binds to lectins or selectins, aiding targeted migration of specific macrophage subsets. CD68's rapid recirculation from endosomes and lysosomes to the plasma membrane allows macrophages to crawl or interact with selectin-bearing substrates and other cells. CD68 Protein, Human (sf9, His) is the recombinant human-derived CD68 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of CD68 Protein, Human (sf9, His) is 319 a.a., with molecular weight of ~68 kDa.
研究背景

CD68 蛋白被认为在组织巨噬细胞进行的各种吞噬活动中发挥着至关重要的作用，包括细胞内溶酶体代谢以及细胞外与细胞和病原体的相互作用。它能够结合组织和器官特异性凝集素或选择素，促进特定巨噬细胞亚群靶向迁移到特定位点。CD68 从内体和溶酶体快速再循环到质膜，使巨噬细胞能够爬过带有选择素的底物或与其他细胞相互作用。
种属

Human
表达系统

Sf9 insect cells
标签

C-His
蛋白编号

P34810 (N22-S319)
基因 ID

968  [NCBI]
蛋白结构
N-term
CD68 (N22-S319)
Accession # P34810
His
C-term
同用名
CD68 antigenmacrophage antigen CD68; CD68; Gp110; Macrosialin; SRD1
分子量

Approximately 68 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

CD68 Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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