1. 重组蛋白
  2. CD Antigens Enzymes & Regulators
  3. B Cell CD Proteins Hydrolases (EC 3)
  4. CD72
  5. CD72 蛋白, Human (Trx, His)

CD72 蛋白, Human (Trx, His)

目录号: HY-P72711
产品使用指南

CD72 蛋白在协调 B 细胞增殖和分化、形成具有二硫键的同二聚体以及与 CD5 相互作用方面发挥着关键作用。它与 PTPN6/SHP-1 进行酪氨酸磷酸化相互作用,表明它通过基于磷酸化的调节机制来调节细胞反应。这强调了 CD72 在 B 细胞功能和信号传导动态景观中的多方面作用。CD72 蛋白, Human (N-Trx-6His) 是重组的 CD72 蛋白,由 E. coli 表达,带有 N-Trx, N-His 标签。CD72 蛋白, Human (N-Trx-6His) 全长 110 个氨基酸,分子量为 26-35 kDa。

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描述

CD72 Protein plays a key role in orchestrating B-cell proliferation and differentiation, forming homodimers with disulfide linkages and interacting with CD5. It engages in tyrosine phosphorylation interactions with PTPN6/SHP-1, indicating its modulation of cellular responses through phosphorylation-based regulatory mechanisms. This underscores the multifaceted role of CD72 in the dynamic landscape of B-cell function and signaling. CD72 Protein, Human (N-Trx-6His) is the recombinant human-derived CD72 protein, expressed by E. coli , with N-Trx, N-His labeled tag. The total length of CD72 Protein, Human (N-Trx-6His) is 110 a.a., with molecular weight of 26-35 kDa.

研究背景

CD72 成为 B 细胞增殖和分化协调的关键参与者。作为具有二硫键的同二聚体,它与 CD5 形成关联,从而在 B 细胞环境中形成复杂的信号网络。此外,CD72 与蛋白质酪氨酸磷酸酶 PTPN6/SHP-1 发生相互作用,特别是酪氨酸磷酸化,表明它通过复杂的基于磷酸化的调节机制参与调节细胞反应。这凸显了 CD72 在 B 细胞功能和信号传导动态景观中的多方面作用。

种属

Human

表达系统

E. coli

标签

N-Trx;N-His

蛋白编号

P21854 (R117-C226)

基因 ID

971  [NCBI]

蛋白结构
N-term
His-Trx
CD72 (R117-C226)
Accession # P21854
C-term
同用名
B-cell differentiation antigen CD72; Lyb-2; CD72
氨基酸序列

RYLQVSQQLQQTNRVLEVTNSSLRQQLRLKITQLGQSAEDLQGSRRELAQSQEALQVEQRAHQAAEGQLQACQADRQKTKETLQSEEQQRRALEQKLSNMENRLKPFFTC

分子量

26-35 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CD72 Protein, Human (Trx, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD72 Protein, Human (Trx, His)
目录号:
HY-P72711
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