CD73/5'-Nucleotidase 蛋白, Mouse (HEK293, His)

CD73/5'-Nucleotidase Protein Protein, Mouse (HEK293, His) is a recombinant mouse CD73 expressed by HEK293 cells with His tag. CD73 is a cell surface enzyme that catalyzes the dephosphorylation of extracellular AMP to adenosine^[1][2].

CD73 是一种糖基磷脂酰肌醇 (GPI) 连接的 70 kDa 细胞表面酶，存在于大多数组织中。CD73 最初被定义为淋巴细胞分化抗原，被认为是 T 淋巴细胞上的协同信号分子，也是对淋巴细胞与内皮细胞结合非常重要的粘附分子^[2]。

Mouse

HEK293

C-6*His

Q61503 (W29-F550)

23959  [NCBI]

WELTILHTNDVHSRLEQTSDDSTKCLNASLCVGGVARLFTKVQQIRKEEPNVLFLDAGDQYQGTIWFTVYKGLEVAHFMNILGYDAMALGNHEFDNGVEGLIDPLLRNVKFPILSANIKARGPLAHQISGLFLPSKVLSVGGEVVGIVGYTSKETPFLSNPGTNLVFEDEISALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDIVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTADDGRQVPVVQAYAFGKYLGYLKVEFDDKGNVITSYGNPILLNSSIPEDATIKADINQWRIKLDNYSTQELGRTIVYLDGSTQTCRFRECNMGNLICDAMINNNLRHPDEMFWNHVSMCIVNGGGIRSPIDEKNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDINRKPWNRVVQLEVLCTKCRVPIYEPLEMDKVYKVTLPSYLANGGDGFQMIKDELLKHDSGDQDISVVSEYISKMKVVYPAVEGRIKF

Approximately 60.0 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filter solution of 20 mM Tris-HCl, 120mM NaCl, 4 mM CaCl₂, 20% Glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Bin Zhang. CD73 promotes tumor growth and metastasis. Oncoimmunology. 2012 Jan 1; 1(1): 67–70.  [Content Brief]

  [2]. Bin Zhang. CD73: A novel target for cancer immunotherapy. Cancer Res. 2010 Aug 15; 70(16): 6407–6411.  [Content Brief]