2. 重组蛋白
  3. Immune Checkpoint Proteins CD Antigens Enzymes & Regulators
  4. Inhibitory Checkpoint Molecules T Cell CD Proteins Macrophage CD Proteins Stem Cell CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins Hydrolases (EC 3)
  5. CD73
  6. CD73/5'-Nucleotidase 蛋白, Human (T376A, HEK293, Fc)

CD73/5'-Nucleotidase 蛋白, Human (T376A, HEK293, Fc)

目录号: HY-P74272
产品使用指南

CD73/5'-核苷酸酶蛋白水解核苷酸单磷酸，有利于 AMP 作为主要底物，并显示出对核糖核苷酸单磷酸的偏好。它释放无机磷酸盐和相应的核苷。CD73 还作用于其他底物，包括 IMP、UMP、GMP、CMP、dAMP、dCMP、dTMP、NAD 和 NMN。CD73/5'-Nucleotidase 蛋白, Human (T376A, HEK293, Fc) 是重组的 CD73/5'-核苷酸酶蛋白，由 HEK293 表达，带有 C-hFc 标签。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
50 μg   询价  
100 μg   询价  

* Please select Quantity before adding items.

Top Publications Citing Use of Products

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

CD73/5'-Nucleotidase Protein hydrolyzes nucleotide monophosphates, favoring AMP as the primary substrate and showing a preference for ribonucleotide monophosphates. It releases inorganic phosphate and the corresponding nucleoside. CD73 also acts on other substrates, including IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD, and NMN. CD73/5'-Nucleotidase Protein, Human (T376A, HEK293, Fc) is the recombinant human-derived CD73/5'-Nucleotidase protein, expressed by HEK293 , with C-hFc labeled tag.
研究背景

CD73/5'-核苷酸酶蛋白通过催化单磷酸核苷酸的水解、释放无机磷酸盐和相应的核苷，在细胞过程中发挥着至关重要的作用。值得注意的是，AMP 是该酶的首选底物，强调了它在腺苷酸核苷酸转化中的重要性。CD73/5'-核苷酸酶表现出对核糖核苷酸单磷酸的偏好超过其脱氧核糖对应物，显示其在底物识别中的选择性。除AMP外，其他底物还包括IMP、UMP、GMP、CMP、dAMP、dCMP、dTMP、NAD和NMN，进一步说明CD73/5'-核苷酸酶在核苷酸代谢中的多功能性及其调节核苷酸平衡的作用细胞内的池。
种属

Human
表达系统

HEK293
标签

C-hFc
蛋白编号

P21589-1/NP_002517.1 (W27-K547, T376A)
基因 ID
蛋白结构
N-term
CD73 (W27-K547, T376A)
Accession # P21589-1/NP_002517.1
hFc
C-term
同用名
5-NT; 5'-Nucleotidase; CD73; E5NT; NT5E; NTE
分子量

Approximately 84.5 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

CD73/5'-Nucleotidase Protein, Human (T376A, HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
CD73/5'-Nucleotidase Protein, Human (T376A, HEK293, Fc)
目录号：
HY-P74272
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z