CD79 蛋白, Mouse (HEK293, His)

CD79A cooperates with CD79B to initiate the B cell antigen receptor complex (BCR) signaling cascade upon antigen binding. This results in complex internalization, transport and antigen presentation. CD79 Protein, Mouse (HEK293, His) is a recombinant protein dimer complex containing mouse-derived CD79 protein, expressed by HEK293 , with C-His labeled tag. CD79 Protein, Mouse (HEK293, His), has molecular weight of 23-43 kDa, respectively.

CD79A 与 CD79B 合作，在启动由抗原与 B 细胞抗原受体复合物 (BCR) 结合触发的信号转导级联中发挥关键作用，从而导致复合物的内化、运输到晚期内体以及随后的抗原呈递。这种不可或缺的蛋白质对于 BCR 表面表达以及原 B 细胞和前 B 细胞的有效分化也至关重要。CD79A不仅刺激SYK自身磷酸化和激活，而且还与BLNK结合，促进BLNK与SYK接近并实现BLNK的磷酸化。此外，它还与特定 Src 家族酪氨酸激酶（包括 FYN 和 LYN）相互作用并增强其活性。在未成熟 B 细胞发育的背景下，CD79A 发挥抑制 BCR 信号传导的作用。作为 α 和 β 链的异二聚体，CD79A 在 B 细胞抗原受体内形成二硫键连接的复合物，其中 α/β 链异二聚体与由两条重链组成的抗原特异性膜结合表面免疫球蛋白非共价结合和两条轻链。通过其磷酸化 ITAM 结构域与 SYK 的 SH2 结构域的相互作用刺激 SYK 自身磷酸化和激活。此外，当 Tyr-204 上磷酸化时，CD79A 与 BLNK/SLP65 的 SH2 结构域相互作用，使 BLNK 与 SYK 接近并促进 BLNK 磷酸化，这是 BCR 运输到晚期核内体的关键步骤。CD79A 的多功能相互作用有助于 B 细胞信号传导和功能的复杂调节。

Measured by its binding ability in a functional ELISA. Immobilized Mouse CD79, at 1μg/mL (100μL/well) can Anti-CD79B antibody. The ED₅₀ for this effect is 63.97 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Mouse CD79, at 1 μg/mL (100 μL/well) can Anti-CD79B antibody. The ED₅₀ for this effect is 63.97 ng/mL.

Mouse

HEK293

C-His

P11911 (L29-R137)&P15530 (V26-D158)

12518  [NCBI]

Approximately 23-43 kDa band in SDS-PAGE under reducing conditions due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.