CD84/SLAMF5 蛋白, Mouse (HEK293, His)

CD84/SLAMF5 is an autoligand receptor of the SLAM family that regulates immune cell activation and differentiation through homotypic or heterotypic interactions. Its activity is affected by SH2D1A/SAP and SH2D1B/EAT-2. CD84/SLAMF5 Protein, Mouse (HEK293, C-His) is the recombinant mouse-derived CD84/SLAMF5 protein, expressed by HEK293 , with C-10*His labeled tag.

CD84 是信号淋巴细胞激活分子 (SLAM) 家族的成员，作为自身配体受体参与免疫反应的复杂调节。通过同型或异型细胞间相互作用，SLAM 受体（包括 CD84）调节不同免疫细胞的激活和分化，有助于协调先天性和适应性免疫反应。CD84 的活性受到小细胞质接头蛋白（例如 SH2D1A/SAP 和 SH2D1B/EAT-2）的存在或不存在的复杂控制。CD84 在自然杀伤 (NK) 细胞的细胞毒性、T 细胞增殖、干扰素 γ (IFNG) 分泌、血小板刺激中发挥作用，并且是造血祖细胞的标记物。此外，CD84 对于 T 细胞：B 细胞相互作用、最佳 T 滤泡辅助功能、生发中心形成以及维持生发中心 B 细胞耐受性以及预防自身免疫至关重要。在肥大细胞中，CD84 负向调节高亲和力免疫球蛋白 ε 受体信号传导，而在巨噬细胞中，它正向调节 LPS 诱导的 MAPK 磷酸化和 NF-kappaB 激活。此外，CD84 通过稳定 IRF8 在增强原代树突状细胞的巨自噬中发挥作用。CD84 通过其胞外结构域形成同型二聚体，并与来自其他细胞的 CD48 分子形成头尾二聚体。它与各种蛋白质相互作用，包括含有 SH2 结构域的蛋白质、酪氨酸蛋白磷酸酶，并受到涉及磷酸化和接头蛋白的复杂调节机制的影响。

Measured in a cell proliferation assay using PHA stimulated Mouse T cells in the presence of anti-CD3. The ED₅₀ for this effect is 0.5203 µg/mL in the presence of anti-CD3 immobilized at 20 ng/mL, corresponding to a specific activity is 1.922×10^3 units/mg.

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  Measured in a cell proliferation assay using PHA stimulated Mouse T cells in the presence of anti-CD3. The ED₅₀ for this effect is 0.5203 µg/mL in the presence of anti-CD3 immobilized at 20 ng/mL, corresponding to a specific activity is 1.922×10³ units/mg.

Mouse

HEK293

C-10*His

Q18PI6-1 (K22-V221)

12523

KDADPMVMNGILGESVTFLLNIQEPKKIDNIAWTSQSSVAFIKPGVNKAEVTITQGTYKGRIEIIDQKYDLVIRDLRMEDAGTYKADINEENEETITKIYYLHIYRRLKTPKITQSLISSLNNTCNITLTCSVEKEEKDVTYSWSPFGEKSNVLQIVHSPMDQKLTYTCTAQNPVSNSSDSVTVQQPCTDTPSFHPRHAV

32-37 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.