1. 重组蛋白
  2. CD Antigens
  3. NK Cell CD Proteins Platelet CD Proteins Erythrocyte CD Proteins Dendritic Cell CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins Cell Adhesion-related CD Proteins
  4. CD99/MIC2
  5. CD99 蛋白, Human (HEK293, Fc)

CD99 蛋白, Human (HEK293, Fc)

目录号: HY-P70752
COA 产品使用指南

CD99 蛋白参与重要的 T 细胞粘附过程,并帮助红细胞自发形成玫瑰花结。它还有助于白细胞外渗的后期阶段,帮助白细胞在免疫反应过程中克服内皮基底膜。CD99 蛋白, Human (HEK293, Fc) 是重组的 CD99 蛋白,由 HEK293 表达,带有 C-hFc 标签。

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规格 价格 是否有货 数量
10 μg ¥1000 In-stock
50 μg ¥2800 In-stock
100 μg   询价  

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描述

The CD99 protein is involved in the important T cell adhesion process and helps red blood cells spontaneously form rosettes. It also contributes to the later stages of leukocyte extravasation, helping leukocytes to overcome the endothelial basement membrane during the immune response. CD99 Protein, Human (HEK293, Fc) is the recombinant human-derived CD99 protein, expressed by HEK293 , with C-hFc labeled tag.

研究背景

CD99 蛋白参与关键的 T 细胞粘附过程,有助于红细胞自发形成玫瑰花结。此外,它在白细胞外渗的后期发挥着至关重要的作用,帮助白细胞在免疫反应过程中克服内皮基底膜。值得注意的是,尽管它作用于同一位点,但其功能独立于 PECAM1。CD99 参与 T 细胞粘附过程强调了其在介导免疫功能所必需的细胞相互作用中的重要性。

种属

Human

表达系统

HEK293

标签

C-hFc

蛋白编号

P14209-1 (D23-D122)

基因 ID
蛋白结构
N-term
CD99 (D23-D122)
Accession # P14209-1
hFc
C-term
同用名
CD99 Antigen; 12E7; E2 Antigen; Protein MIC2; T-Cell Surface Glycoprotein E2; CD99; MIC2; MIC2X; MIC2Y
氨基酸序列

DGGFDLSDALPDNENKKPTAIPKKPSAGDDFDLGDAVVDGENDDPRPPNPPKPMPNPNPNHPSSSGSFSDADLADGVSGGEGKGGSDGGGSHRKEGEEAD

分子量

Approximately 58.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD99 Protein, Human (HEK293, Fc)
目录号:
HY-P70752
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