2. 重组蛋白
  3. CD Antigens
  4. NK Cell CD Proteins Platelet CD Proteins Erythrocyte CD Proteins Dendritic Cell CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins Cell Adhesion-related CD Proteins
  5. CD99/MIC2
  6. CD99 蛋白, Human (HEK293, His)

CD99 蛋白, Human (HEK293, His)

目录号: HY-P77623
产品使用指南

CD99 蛋白参与重要的 T 细胞粘附过程，并帮助红细胞自发形成玫瑰花结。它还有助于白细胞外渗的后期阶段，帮助白细胞在免疫反应过程中克服内皮基底膜。CD99 蛋白, Human (HEK293, His) 是重组的 CD99 蛋白，由 HEK293 表达，带有 N-His 标签。

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描述

The CD99 protein is involved in the important T cell adhesion process and helps red blood cells spontaneously form rosettes. It also contributes to the later stages of leukocyte extravasation, helping leukocytes to overcome the endothelial basement membrane during the immune response. CD99 Protein, Human (HEK293, His) is the recombinant human-derived CD99 protein, expressed by HEK293 , with N-His labeled tag.
研究背景

CD99 蛋白参与关键的 T 细胞粘附过程，有助于红细胞自发形成玫瑰花结。此外，它在白细胞外渗的后期发挥着至关重要的作用，帮助白细胞在免疫反应过程中克服内皮基底膜。值得注意的是，尽管它作用于同一位点，但其功能独立于 PECAM1。CD99 参与 T 细胞粘附过程强调了其在介导免疫功能所必需的细胞相互作用中的重要性。
种属

Human
表达系统

HEK293
标签

N-His
蛋白编号

P14209-1 (D23-D122)
基因 ID
蛋白结构
N-term
His
CD99 (D23-D122)
Accession # P14209-1
C-term
同用名
12E7; E2 antigen; MIC2; MIC2X; MIC2Y; CD99; HBA71; MSK5X; 12E7; 1110061M03Rik; 2410026K10Rik; D4; pilr-1; Pilr-l; CD99 molecule
分子量

20-25 kDa
纯度

Greater than 95% as determined by Tris-Bis PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

CD99 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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