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CDC37 蛋白, Mouse (sf9, His-GST)

目录号: HY-P75362
产品使用指南

CDC37 蛋白是一种共伴侣,可与激酶结合并促进激酶与 Hsp90 复合物的相互作用,从而稳定和激活它们。它抑制 HSP90AA1 的 ATP 酶活性。CDC37 蛋白, Mouse (sf9, His-GST) 是重组的 CDC37 蛋白,由 Sf9 insect cells 表达,带有 N-His, N-GST 标签。CDC37 蛋白, Mouse (sf9, His-GST) 全长 379 个氨基酸,分子量约为 ~66 kDa。

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描述

The CDC37 protein is a co-chaperone that binds to kinases and promotes their interaction with Hsp90 complexes, thereby stabilizing and activating them. It inhibits the ATPase activity of HSP90AA1. CDC37 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived CDC37 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of CDC37 Protein, Mouse (sf9, His-GST) is 379 a.a., with molecular weight of ~66 kDa.

研究背景

CDC37 蛋白是一种共伴侣,可与各种激酶结合并促进它们与 Hsp90 复合物的相互作用,从而稳定和激活这些激酶。它抑制 HSP90AA1 的 ATP 酶活性。它可能形成由伴侣 HSP90 和 HSP70、辅助伴侣 STIP1/HOP、CDC37、PPP5C、PTGES3/p23、TSC1 和客户蛋白 TSC2 组成的复合物。该复合体还可能包括 CDK4、AKT、RAF1 和 NR3C1,但不包含 STIP1/HOP 和 PTGES3/p23。CDC37 与 Hsp90/HSP90AB1 和 CDK6 形成单独的复合物。它与多种蛋白质相互作用,包括 HSP90AA1、AR、CDK4、CDK6、EIF2AK1、RB1、KSR1、FLCN、FNIP1 和 FNIP2。

种属

Mouse

表达系统

Sf9 insect cells

标签

N-His;N-GST

蛋白编号

Q61081 (M1-A379)

基因 ID
蛋白结构
N-term
His-GST
CDC37 (M1-A379)
Accession # Q61081
C-term
同用名
Hsp90 co-chaperone Cdc37; CDC37; CDC37A
分子量

Approximately 66 kDa

纯度

Greater than 85% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CDC37 Protein, Mouse (sf9, His-GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CDC37 Protein, Mouse (sf9, His-GST)
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HY-P75362
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