CDK12-CCNK Heterodimer 蛋白, Human (Sf9, GST, FLAG)

The CDK12 protein is a cyclin-dependent kinase that critically phosphorylates the C-terminal domain of POLR2A, thereby regulating transcription elongation. It selectively targets "Ser-5" phosphorylated at "Ser-7" in the CTD repeat and can also target "Ser-2". CDK12-CCNK Heterodimer Protein, Human (Sf9, GST, FLAG) is a recombinant protein dimer complex containing human-derived CDK12-CCNK Heterodimer protein, expressed by Sf9 insect cells , with N-Flag, N-GST labeled tag.

CDK12-CCNK 异二聚体蛋白作为细胞周期蛋白依赖性激酶发挥作用，通过磷酸化 RNA 聚合酶 II (POLR2A) 大亚基的 C 末端结构域 (CTD)，在转录延伸中发挥至关重要的作用。该激酶是基因表达的关键调节因子，特别是参与 DNA 修复过程和基因组稳定性的维持。其磷酸化活性是选择性的，优先选择 CTD 重复序列中已在“Ser-7”处磷酸化的“Ser-5”，但它也可以靶向“Ser-2”。CDK12-CCNK 对于 RNA 剪接至关重要，可能通过 SRSF1/SF2 的磷酸化实现。此外，它还参与 MAP 激酶活性的调节，表明它参与影响对雌激素抑制剂的反应。

The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. The Km of CDK12-CCNK for its substrate is 30 μM.

Human

Sf9 insect cells

N-Flag;N-GST

Q9NYV4-1 (Q696-S1082)&O75909-1 (M1-S300)

51755&8812

71.4 kDa & 36.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 20 mM HEPES, pH 7.5, 200 mM NaCl, 5% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.