CDK2 蛋白, Human (His)

CDK2 is an important serine/threonine protein kinase that plays a critical regulatory role in the cell cycle, especially during meiosis. It phosphorylates various substrates (CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2) and coordinates centrosome duplication and initiation of DNA synthesis at the G1-S transition. CDK2 Protein, Human (His) is the recombinant human-derived CDK2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of CDK2 Protein, Human (His) is 298 a.a., with molecular weight of 32-35 kDa.

CDK2 是一种丝氨酸/苏氨酸蛋白激酶，复杂地调节细胞周期，在减数分裂中发挥着不可或缺的作用，但对于有丝分裂来说却是可有可无的。CDK2 在细胞过程的联系中发挥作用，磷酸化多种底物，包括 CTNNB1、USP37、p53/TP53、NPM1、CDK7、RB1、BRCA2、MYC、NPAT 和 EZH2。它协调关键事件，例如 G1-S 转变时的中心体复制和 DNA 合成起始，调节 G2 进展并微调人类胚胎干细胞中细胞增殖、死亡和 DNA 修复之间的平衡。CDK2 依次被细胞周期蛋白 E 和细胞周期蛋白 A2 激活，调节从 S 期到有丝分裂的转变。值得注意的是，EZH2 的磷酸化与维持 H3K27me3 和表观遗传基因沉默有关。CDK2 的参与延伸到 DNA 损伤反应，它调节 G1-S 期检查点、磷酸化 BRCA2 并调节同源重组依赖性修复。此外，CDK2 参与多种信号传导途径，包括维生素 D 介导的生长抑制、NO 介导的信号传导以及 FOXP3 和 ERCC6 等底物的磷酸化，突出了其在细胞生理学中的多方面作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

P24941 (E2-L298)

1017  [NCBI]

ENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL

32-35 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl/200 mM Tris, 200 mM NaCl, 1 mM DTT, 40% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.