1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. CDK3-CCNE2 蛋白, Human (sf9, GST, Flag, His)

CDK3-CCNE2 蛋白, Human (sf9, GST, Flag, His)

目录号: HY-P702681
产品使用指南

CDK3蛋白是一种丝氨酸/苏氨酸蛋白激酶,在调节真核细胞周期中发挥关键作用,特别是影响G0-G1和G1-S转变。它在间期与 CCNC/cyclin-C 相互作用,并磷酸化组蛋白 H1、ATF1、RB1 和 CABLES1 等底物。CDK3-CCNE2 蛋白, Human (sf9, GST, Flag, His) 是重组的 CDK3-CCNE2 蛋白,由 Sf9 insect cells 表达,带有 N-His, N-GST, N-Flag 标签。CDK3-CCNE2 蛋白, Human (sf9, GST, Flag, His)

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     可免费申领三个不同产品的试用装。

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描述

CDK3 protein is a serine/threonine protein kinase that plays a key role in regulating the eukaryotic cell cycle, especially affecting the G0-G1 and G1-S transitions. It interacts with CCNC/cyclin-C during interphase and phosphorylates substrates such as histone H1, ATF1, RB1, and CABLES1. CDK3-CCNE2 Protein, Human (sf9, GST, Flag, His) is the recombinant human-derived CDK3-CCNE2, expressed by Sf9 insect cells , with N-His, N-GST, N-Flag labeled tag. ,

研究背景

CDK3 蛋白是一种丝氨酸/苏氨酸蛋白激酶,在真核细胞周期的调节中发挥着关键作用,特别是影响 G0-G1 和 G1-S 转变。在间期,它与 CCNC/cyclin-C 相互作用。该激酶磷酸化关键底物,包括组蛋白 H1、ATF1、RB1 和 CABLES1。CDK3-CCNE1 诱导的 ATF1 磷酸化触发 ATF1 反式激活和转录活性,从而促进细胞增殖和转化。此外,CDK3/cyclin-C 介导的 RB1 磷酸化对于 G0-G1 转变至关重要。值得注意的是,CDK3-CCNE1 可能通过以独立于 RB1 的方式参与 E2F1、E2F2 和 E2F3 的激活来促进 G1-S 转变。这种复杂的相互作用使 CDK3-CCNE1 成为细胞周期进程的关键协调者。

种属

Human

表达系统

Sf9 insect cells

标签

N-His;N-GST;N-Flag

蛋白编号

Q00526 (M1-H305)&O96020-1 (S2-H404 )

基因 ID

1018&9134

同用名
CDK3; CCNE1; Cyclin-dependent kinase 3; Cell division protein kinase 3; G1/S-specific cyclin-E1
分子量

61.6 kDa&49.4 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CDK3-CCNE2 Protein, Human (sf9, GST, Flag, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CDK3-CCNE2 Protein, Human (sf9, GST, Flag, His)
目录号:
HY-P702681
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