2. 重组蛋白
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. CELA2A 蛋白, Mouse (His)

CELA2A 蛋白, Mouse (His)

目录号: HY-P72136
产品使用指南

CELA2A 蛋白是一种丝氨酸蛋白酶，参与小肠中膳食蛋白质的消化。它裂解肽键，将蛋白质分解成更小的肽和氨基酸以供吸收。CELA2A 蛋白, Mouse (His) 是重组的 CELA2A 蛋白，由 E. coli 表达，带有 N-6*His 标签。CELA2A 蛋白, Mouse (His) 全长 241 个氨基酸，分子量约为 ~29.7 kDa。

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规格 是否有货
50 μg   询价  
100 μg   询价  

* Please select Quantity before adding items.

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描述

CELA2A Protein, a serine protease, is involved in the digestion of dietary proteins in the small intestine. It cleaves peptide bonds, breaking down proteins into smaller peptides and amino acids for absorption. CELA2A Protein, Mouse (His) is the recombinant mouse-derived CELA2A protein, expressed by E. coli , with N-6*His labeled tag. The total length of CELA2A Protein, Mouse (His) is 241 a.a., with molecular weight of ~29.7 kDa.
研究背景

该蛋白参与调节先天免疫和 IFN-γ 引起的炎症反应。它通过充当组装自噬相关的各种蛋白质的平台来组织自噬机制。它还识别特定的自噬目标并协调目标识别与自噬的启动。
种属

Mouse
表达系统

E. coli
标签

N-6*His
蛋白编号

P05208 (V31-N271)
基因 ID
蛋白结构
N-term
6*His
CELA2A (V31-N271)
Accession # P05208
C-term
同用名
Cela2a; Ela-2; Ela2; Ela2aChymotrypsin-like elastase family member 2A; EC 3.4.21.71; Elastase-2; Elastase-2A
氨基酸序列

VVGGQEATPNTWPWQVSLQVLSSGRWRHNCGGSLVANNWVLTAAHCLSNYQTYRVLLGAHSLSNPGAGSAAVQVSKLVVHQRWNSQNVGNGYDIALIKLASPVTLSKNIQTACLPPAGTILPRNYVCYVTGWGLLQTNGNSPDTLRQGRLLVVDYATCSSASWWGSSVKSSMVCAGGDGVTSSCNGDSGGPLNCRASNGQWQVHGIVSFGSSLGCNYPRKPSVFTRVSNYIDWINSVMARN

分子量

Approximately 29.7 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

CELA2A Protein, Mouse (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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