2. 重组蛋白
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. CELA3A 蛋白, Human (HEK293, His)

CELA3A 蛋白, Human (HEK293, His)

目录号: HY-P70133
COA 产品使用指南

CELA3A 蛋白是一种高效的丙氨酸特异性蛋白酶，具有最小的弹力分解活性。CELA3A 蛋白, Human (HEK293, His) 是重组的 CELA3A 蛋白，由 HEK293 表达，带有 C-6*His 标签。CELA3A 蛋白, Human (HEK293, His) 全长 255 个氨基酸，分子量约为 ~31.0 kDa。

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
10 μg ￥1500 In-stock
50 μg ￥4200 In-stock
100 μg   询价  

* Please select Quantity before adding items.

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描述

CELA3A Protein, an efficient alanine-specific protease, exhibits minimal elastolytic activity. CELA3A Protein, Human (HEK293, His) is the recombinant human-derived CELA3A protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CELA3A Protein, Human (HEK293, His) is 255 a.a., with molecular weight of ~31.0 kDa.
研究背景

CELA3A 蛋白作为一种有效的蛋白酶，对丙氨酸具有特异性，表现出有限的弹力分解活性。
生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
种属

Human
表达系统

HEK293
标签

C-6*His
蛋白编号

P09093 (S16-H270)
基因 ID
蛋白结构
N-term
CELA3A (S16-H270)
Accession # P09093
6*His
C-term
同用名
rHuChymotrypsin-like elastase family member 3A/CELA3A, His; Chymotrypsin-Like Elastase Family Member 3A; Elastase IIIA; Elastase-3A; Protease E; CELA3A; ELA3; ELA3A
氨基酸序列

SGYGPPSSHSSSRVVHGEDAVPYSWPWQVSLQYEKSGSFYHTCGGSLIAPDWVVTAGHCISRDLTYQVVLGEYNLAVKEGPEQVIPINSEELFVHPLWNRSCVACGNDIALIKLSRSAQLGDAVQLASLPPAGDILPNKTPCYITGWGRLYTNGPLPDKLQQARLPVVDYKHCSRWNWWGSTVKKTMVCAGGYIRSGCNGDSGGPLNCPTEDGGWQVHGVTSFVSAFGCNFIWKPTVFTRVSAFIDWIEETIASH

分子量

Approximately 31.0 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
性状

溶液.
组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 10% Glycerol, pH 7.5.
内毒素含量

<1 EU/μg, determined by LAL method.
保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
运输条件

Shipping with dry ice.
文件资料

COA (209 KB)

产品使用指南 (1213 KB)

CELA3A Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CELA3A Protein, Human (HEK293, His)
目录号：
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