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  3. Cerberus 1/CER1 蛋白, Human (HEK293, His)

Cerberus 1/CER1 蛋白, Human (HEK293, His)

目录号: HY-P7822
COA 产品使用指南

CER1 蛋白,是 DAN 家族 BMP 拮抗剂,通过诱导 BMP 抑制而协调促进生长的输尿管芽信号。CER1 还通过 ALK4/ALK7 抑制 NODAL 信号转导至 SMAD2SMAD3,从而调控胚胎发生和癌变。Cerberus 1/CER1 蛋白, Human (HEK293, His) 是重组的 Cerberus CER1 蛋白,由 HEK293 表达,带有 C-6*His 标签。Cerberus 1/CER1 蛋白, Human (HEK293, His) 全长 250 个氨基酸,分子量为 35-42 kDa。

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规格 价格 是否有货 数量
10 μg ¥1400 In-stock
50 μg ¥3800 询价
100 μg   询价  

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描述

The CER1 protein, a DAN family BMP antagonist, coordinates growth-promoting ureteric bud signaling by inducing BMP inhibition. CER1 also regulates embryogenesis and carcinogenesis by inhibiting NODAL signaling to SMAD2 or SMAD3 through ALK4/ALK7. Cerberus 1/CER1 Protein, Human (HEK293, His) is the recombinant human-derived Cerberus 1/CER1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Cerberus 1/CER1 Protein, Human (HEK293, His) is 250 a.a., with molecular weight of 35-42 kDa.

研究背景

Cerberus 1, DAN family BMP antagonist (CER1),是半胱氨酸结超家族蛋白同源物和 DAN 家族 BMP 拮抗剂。CER1 具有九个保守的半胱氨酸和一个半胱氨酸结区域,与 Dan 和 DRM/Gremlin 代表骨形态发生蛋白 (BMP) 拮抗剂,可以直接与 BMP 结合并抑制其活性。人 ES 细胞中 CER1 的上调导致与人 ES 细胞分化相关的 Nodal 信号传导抑制。CER1 (Cerberus 1) 和 GREM3(CKTSF1B3 或 CER2)通过 ACVR1B (ALK4) 或 ACVR1C (ALK7) 抑制 NODAL 信号转导至 SMAD2SMAD3。而 Nodal 和 BMP 信号通路与 WNT 信号通路形成的网络与胚胎发生和癌变过程有关。尽管,CER1 在一定程度上抑制了 Wnt3a 诱导的经典 Top Flash 报告基因,但它并不影响细胞培养中的 Wnt11 信号传导。并且,CER1 通过 Bmp 介导的拮抗作用以及在某种程度上通过参与分支的典型 Wnt 信号来协调促进生长的输尿管芽信号 Gndf 和 Wnt11 的活动,从而微调输尿管树的空间组织。

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

AAI03977.1 (T18-A267)

基因 ID
蛋白结构
N-term
CER1 (T18-A267)
Accession # AAI03977.1
6*His
C-term
同用名
rHuCerberus 1, cysteine knot superfamily, homolog (Xenopus laevis)/CER1, His; Cerberus; Cerberus-Related Protein; DAN Domain Family Member 4; CER1; DAND4
氨基酸序列

TRHQDGRQNQSSLSPVLLPRNQRELPTGNHEEAEEKPDLFVAVPHLVGTSPAGEGQRQREKMLSRFGRFWKKPEREMHPSRDSDSEPFPPGTQSLIQPIDGMKMEKSPLREEAKKFWHHFMFRKTPASQGVILPIKSHEVHWETCRTVPFSQTITHEGCEKLVVQNNLCFGKCGSVHFPGAAQHSHTSCSHCLPAKFTTMHLPLNCTELSSVIKVVMLVEECQCKVKTEHEDGHILHAGSQDSFIPGVSA

分子量

35-42 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM NaAc-HAC, pH 4.5.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Cerberus 1/CER1 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Cerberus 1/CER1 Protein, Human (HEK293, His)
目录号:
HY-P7822
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