1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. CES2/carboxylesterase 2 蛋白, Human (HEK293,C-His)

CES2/carboxylesterase 2 蛋白, Human (HEK293,C-His)

目录号: HY-P76192
COA 产品使用指南

CES2 蛋白在异生素解毒以及激活酯和酰胺前药方面发挥着至关重要的作用。它具有很高的催化效率,特别是在可卡因、4-甲基伞形乙酸酯、海洛因和6-单乙酰吗啡等物质的水解方面。CES2/carboxylesterase 2 蛋白, Human (HEK293,C-His) 是重组的 CES2/羧酸酯酶 2 蛋白,由 HEK293 表达,带有 C-His 标签。CES2/carboxylesterase 2 蛋白, Human (HEK293,C-His) 全长 533 个氨基酸,分子量约为 ~60.4 KDa。

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规格 价格 是否有货 数量
50 μg ¥5200 In-stock
100 μg   询价  

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CES2/carboxylesterase 2 Protein, Human (HEK293,C-His) 相关产品:

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The CES2 protein plays a crucial role in detoxifying xenobiotics and activating ester and amide prodrugs. It has high catalytic efficiency, especially in the hydrolysis of substances such as cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine. CES2/carboxylesterase 2 Protein, Human (HEK293,C-His) is the recombinant human-derived CES2/carboxylesterase 2 protein, expressed by HEK293 , with C-His labeled tag. The total length of CES2/carboxylesterase 2 Protein, Human (HEK293,C-His) is 533 a.a., with molecular weight of ~60.4 KDa.

研究背景

CES2/羧酸酯酶 2 蛋白在外源物质的解毒以及酯和酰胺前药的激活中发挥着至关重要的作用。它在多种化合物的水解中表现出高催化效率,包括可卡因、4-甲基伞形基乙酸酯、海洛因和 6-单乙酰吗啡。此外,CES2 还可以水解阿司匹林、具有大醇基和小酰基的底物,以及内源性脂质,例如三酰甘油。值得注意的是,它参与单酰基甘油酯向游离脂肪酸和甘油的转化。此外,CES2 还表现出 2-花生四烯酰甘油和前列腺素水解活性,突出了其酶功能的多功能性,对各种生物过程和药物代谢具有潜在影响。

生物活性

Measured by its ability to hydrolyze p-nitrophenylacetate.The specific activity is >20000 pmols/min/μg.

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

O00748-1 (Q27-L559)

基因 ID
蛋白结构
N-term
CES2 (Q27-L559)
Accession # O00748-1
His
C-term
同用名
Cocaine esterase; Carboxylesterase 2; CE-2; CES2; ICE
氨基酸序列

QDSASPIRTTHTGQVLGSLVHVKGANAGVQTFLGIPFAKPPLGPLRFAPPEPPESWSGVRDGTTHPAMCLQDLTAVESEFLSQFNMTFPSDSMSEDCLYLSIYTPAHSHEGSNLPVMVWIHGGALVFGMASLYDGSMLAALENVVVVIIQYRLGVLGFFSTGDKHATGNWGYLDQVAALRWVQQNIAHFGGNPDRVTIFGESAGGTSVSSLVVSPISQGLFHGAIMESGVALLPGLIASSADVISTVVANLSACDQVDSEALVGCLRGKSKEEILAINKPFKMIPGVVDGVFLPRHPQELLASADFQPVPSIVGVNNNEFGWLIPKVMRIYDTQKEMDREASQAALQKMLTLLMLPPTFGDLLREEYIGDNGDPQTLQAQFQEMMADSMFVIPALQVAHFQCSRAPVYFYEFQHQPSWLKNIRPPHMKADHGDELPFVFRSFFGGNYIKFTEEEEQLSRKMMKYWANFARNGNPNGEGLPHWPLFDQEEQYLQLNLQPAVGRALKAHRLQFWKKALPQKIQELEEPEERHTEL

分子量

Approximately 60.4 kDa.

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 50 mM NaAc, 150 mM NaCl, 10% Glycerol, pH 5.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

CES2/carboxylesterase 2 Protein, Human (HEK293,C-His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CES2/carboxylesterase 2 Protein, Human (HEK293,C-His)
目录号:
HY-P76192
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