1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. CES2/carboxylesterase 2 蛋白, Mouse (HEK293, His)

CES2/carboxylesterase 2 蛋白, Mouse (HEK293, His)

目录号: HY-P76193
COA 产品使用指南

CES2 是一种水解酶,对棕榈酰肉碱和其他底物(如对硝基苯乙酸酯和 α-萘基乙酸酯)表现出显着的活性。其多功能酶催化能力延伸至视黄酯的水解。CES2/carboxylesterase 2 蛋白, Mouse (HEK293, His) 是重组的 CES2/羧酸酯酶 2 蛋白,由 HEK293 表达,带有 C-His 标签。

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10 μg ¥1450 In-stock
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100 μg ¥6000 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

CES2, a hydrolase, demonstrates notable activity with palmitoylcarnitine and other substrates like p-nitrophenylacetate and alpha-naphthylacetate. Its versatile enzymatic capabilities extend to the hydrolysis of retinyl esters. CES2/carboxylesterase 2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CES2/carboxylesterase 2 protein, expressed by HEK293 , with C-His labeled tag.

研究背景

CES2 是一种水解酶,对棕榈酰肉碱表现出显着的活性,并且对乙酸对硝基苯酯和乙酸萘酯等底物表现出额外的酶功效。此外,CES2 可能参与视黄酯的水解,展示其多功能的酶促能力。

生物活性

Measured by its ability to hydrolyze pnitrophenylacetate. The specific activity is ≥75,589.342 pmol/min/μg.

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

Q91WG0 (Q27-K557)

基因 ID
蛋白结构
N-term
CES2 (Q27-K557)
Accession # Q91WG0
His
C-term
同用名
Cocaine esterase; Carboxylesterase 2; CE-2; CES2; ICE
氨基酸序列

QDSPEANPIRNTHTGQIQGSLIHVKDTKAGVHTFLGIPFAKPPVGPLRFAPPEAPEPWSGVRDGTAHPAMCLQNLDMLNEAGLPDMKMMLSSFPMSEDCLYLNIYTPAHAHEGSNLPVMVWIHGGALVIGMASMFDGSLLTVNEDLVVVTIQYRLGVLGFFSTGDQHARGNWGYLDQAAALRWVQQNIAHFGGNPDRVTIFGESAGGTSVSSHVVSPMSQGLFHGAIMESGVALLPDLISETSEMVSTTVAKLSGCEAMDSQALVRCLRGKSEAEILAINKVFKMIPAVVDGEFFPRHPKELLASEDFHPVPSIIGVNNDEFGWSIPVVMGSAQMIKGITRENLQAVLKDTAVQMMLPPECSDLLMEEYMGDTEDAQTLQIQFTEMMGDFMFVIPALQVAHFQRSHAPVYFYEFQHPPSYFKDVRPPHVKADHADEIPFVFASFFWGMKLDFTEEEELLSRRMMKYWANFARHGNPNSEGLPYWPVMDHDEQYLQLDIQPAVGRALKAGRLQFWTKTLPQKIQELKASQDK

分子量

Approximately 52-60 kDa.

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

CES2/carboxylesterase 2 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CES2/carboxylesterase 2 Protein, Mouse (HEK293, His)
目录号:
HY-P76193
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