1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Peptide Hormone & Neuropeptides
  4. CG-alpha
  5. CG alpha 蛋白, Human (HEK293)

CG alpha 蛋白, Human (HEK293)

目录号: HY-P75342
COA 产品使用指南

CG α 是一种糖蛋白激素亚基,有助于产生绒毛膜促性腺激素、黄体生成素、促卵泡激素和促甲状腺激素。虽然 α 亚基相同,但 β 链具有特异性。CG α 由该基因编码,属于糖蛋白激素 α 链家族。在胎盘中限制表达 (RPKM 388.3)。存在两种转录变体。CG alpha 蛋白, Human (HEK293) 是重组的 CG α 蛋白,由 HEK293 表达,不带标签。CG alpha 蛋白, Human (HEK293) 全长 92 个氨基酸,分子量分别约为 ~11.86 & 15-22 kDa。

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描述

CG alpha, a glycoprotein hormone subunit, contributes to chorionic gonadotropin, luteinizing hormone, follicle-stimulating hormone, and thyroid-stimulating hormone. While alpha subunits are identical, beta chains confer specificity. Encoded by this gene, CG alpha belongs to the glycoprotein hormones alpha chain family. Restrictedly expressed in the placenta (RPKM 388.3). Two transcript variants exist. CG alpha Protein, Human (HEK293) is the recombinant human-derived CG alpha protein, expressed by HEK293 , with tag free. The total length of CG alpha Protein, Human (HEK293) is 92 a.a., with molecular weight of ~11.86 & 15-22 kDa, respectively.

研究背景

CG α 蛋白是人类糖蛋白激素(包括绒毛膜促性腺激素 (CG)、黄体生成素 (LH)、卵泡刺激素 (FSH) 和甲状腺刺激素 (TSH))编码的 α 亚基,在形成中发挥着关键作用具有β亚基的非共价二聚体,赋予每种激素生物特异性。虽然这些激素的 α 亚基是相同的,但它们独特的 β 链决定了它们独特的功能。CG α 属于糖蛋白激素 α 链家族,主要在胎盘中表达 (RPKM 388.3)。限制性表达模式突出了其在胎盘组织中的专门性,在胎盘组织中它有助于对生殖和内分泌过程至关重要的糖蛋白激素的调节功能。已鉴定出该基因编码不同亚型的两种转录变体,增加了其在各种生理背景下调节机制的复杂性。

种属

Human

表达系统

HEK293

标签

Tag Free

蛋白编号

NP_000726.1 (A25-S116)

基因 ID
蛋白结构
N-term
CG alpha (A25-S116)
Accession # NP_000726.1
C-term
同用名
Glycoprotein hormones alpha chain; CG-alpha; FSH-alpha; CGA
氨基酸序列

APDVQDCPECTLQENPFFSQPGAPILQCMGCCFSRAYPTPLRSKKTMLVQKNVTSESTCCVAKSYNRVTVMGGFKVENHTACHCSTCYYHKS

分子量

Approximately 11.86&15-22 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

CG alpha Protein, Human (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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