CHI3L1 蛋白, Mouse (HEK293, His)

The CHI3L1 protein is a carbohydrate-binding lectin with a preference for chitin that is involved in tissue remodeling and cellular adaptation to environmental changes. It is involved in Th2 inflammation, IL-13-induced inflammation, allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation, M2 macrophage differentiation and pathogenic intestinal bacterial invasion. CHI3L1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CHI3L1 protein, expressed by HEK293 , with C-10*His labeled tag.

CHI3L1 蛋白是一种碳水化合物结合凝集素，对几丁质表现出特定的偏好。尽管它缺乏几丁质酶活性，但它在组织重塑中发挥着至关重要的作用，并使细胞能够适应和响应环境变化。它参与多种过程，例如2型辅助性T细胞（Th2）炎症反应、IL-13诱导的炎症、过敏原致敏的调节、炎症细胞凋亡的调节、树突状细胞积累和促进M2巨噬细胞分化。此外，它还促进致病性肠道细菌侵入结肠粘膜和淋巴器官。在结肠上皮细胞中，它介导 AKT1 信号通路的激活，导致随后产生 IL8。CHI3L1 还通过增强巨噬细胞杀灭细菌、控制细菌传播和促进宿主耐受来调节肺部的抗菌反应。此外，它还在调节肺中高氧诱导的损伤、炎症和上皮细胞凋亡中发挥作用。它以单体形式存在。

Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. The ED₅₀ for this effect is 3.232 μg/ml, corresponding to a specific activity is 309.4059 units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. The ED₅₀ for this effect is 3.232 μg/mL, corresponding to a specific activity is 309.4059 units/mg.

Mouse

HEK293

C-10*His

Q61362-2 (Y22-A381)

12654  [NCBI]

YKLVCYFTSWSQYREGVGSFLPDAIQPFLCTHIIYSFANISSDNMLSTWEWNDESNYDKLNKLKTRNTNLKTLLSVGGWKFGEKRFSEIASNTERRTAFVRSVAPFLRSYGFDGLDLAWLYPRLRDKQYFSTLIKELNAEFTKEVQPGREKLLLSAALSAGKVAIDTGYDIAQIAQHLDFINLMTYDFHGVWRQITGHHSPLFQGQKDTRFDRYSNVNYAVQYMIRLGAQASKLLMGIPTFGKSFTLASSENQLGAPISGEGLPGRFTKEAGTLAYYEICDFLKGAEVHRLSNEKVPFATKGNQWVGYEDKESVKNKVGFLKEKKLAGAMVWALDLDDFQGTCQPKEFFPLTNAIKDALA

Approximately 40.83 kDa

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  Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.