Chitinase-like 蛋白 3/YM1 蛋白, Mouse (HEK293, His)

Chitinase-like protein 3/YM1, functioning as a lectin, demonstrates a strong affinity for saccharides containing free amino groups, particularly oligomeric saccharides. It binds to chitin and heparin, displaying weak hexosaminidase activity without chitinase activity. Notably, the protein exhibits chemotactic activity for T-lymphocytes, bone marrow cells, and eosinophils, indicating potential roles in inflammation and allergies. Chitinase-like protein 3/YM1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Chitinase-like protein 3/YM1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Chitinase-like protein 3/YM1 Protein, Mouse (HEK293, His) is 377 a.a., with molecular weight of 41-45 kDa.

几丁质酶样蛋白 3/YM1 蛋白起到凝集素的作用，对含有游离氨基的糖类（包括葡萄糖胺或半乳糖胺）具有显着的亲和力。与单糖或二糖相比，其对低聚糖的结合亲和力要强得多。此外，几丁质酶样蛋白 3/YM1 与几丁质和肝素结合。虽然它表现出弱己糖胺酶活性，但它不具有几丁质酶活性。除了其酶促功能外，该蛋白还对 T 淋巴细胞、骨髓细胞和嗜酸性粒细胞表现出趋化活性，表明其可能参与炎症和过敏过程。

Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. The ED₅₀ for this effect is 0.9299 μg/mL, corresponding to a specific activity is 1075.3844 units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. The ED₅₀ for this effect is 0.9299 μg/mL, corresponding to a specific activity is 1075.3844 units/mg.

Mouse

HEK293

C-6*His

O35744 (Y22-Y398)

12655  [NCBI]

YQLMCYYTSWAKDRPIEGSFKPGNIDPCLCTHLIYAFAGMQNNEITYTHEQDLRDYEALNGLKDKNTELKTLLAIGGWKFGPAPFSAMVSTPQNRQIFIQSVIRFLRQYNFDGLNLDWQYPGSRGSPPKDKHLFSVLVKEMRKAFEEESVEKDIPRLLLTSTGAGIIDVIKSGYKIPELSQSLDYIQVMTYDLHDPKDGYTGENSPLYKSPYDIGKSADLNVDSIISYWKDHGAASEKLIVGFPAYGHTFILSDPSKTGIGAPTISTGPPGKYTDESGLLAYYEVCTFLNEGATEVWDAPQEVPYAYQGNEWVGYDNVRSFKLKAQWLKDNNLGGAVVWPLDMDDFSGSFCHQRHFPLTSTLKGDLNIHSASCKGPY

41-45 kDa

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  Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.