CHRNA1 蛋白, Mouse (His)

The CHRNA1 protein, also known as the acetylcholine receptor (AChR), undergoes significant conformational changes upon binding of acetylcholine. This change affects all subunits, causing the opening of ion-conducting channels across the plasma membrane. CHRNA1 Protein, Mouse (His) is the recombinant mouse-derived CHRNA1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of CHRNA1 Protein, Mouse (His) is 210 a.a., with molecular weight of ~25 kDa.

CHRNA1 蛋白，也称为乙酰胆碱受体 (AChR) 的 alpha-1 亚基，在介导乙酰胆碱结合的细胞反应中发挥着至关重要的作用。作为 AChR 的组成部分，它是由两条 α 链、一条 β、一条 δ 以及一条 γ（在未成熟肌肉中）或 ε（在成熟肌肉中）链组成的五聚体结构的一部分。乙酰胆碱结合后，AChR 的所有亚基都会发生广泛的构象变化，导致跨质膜的离子传导通道打开。这个过程是神经肌肉接头处神经信号传输不可或缺的一部分。此外，包含 α-1、β-1、δ 和 ε 亚基的肌肉异五聚体与 α-芋螺毒素 ImII 相互作用，进一步凸显了乙酰胆碱受体复合物内 CHRNA1 功能所涉及的复杂分子相互作用。

Mouse

E. coli

N-6*His

P04756 (S21-L230)

11435  [NCBI]

SEHETRLVAKLFEDYSSVVRPVEDHREIVQVTVGLQLIQLINVDEVNQIVTTNVRLKQQWVDYNLKWNPDDYGGVKKIHIPSEKIWRPDVVLYNNADGDFAIVKFTKVLLDYTGHITWTPPAIFKSYCEIIVTHFPFDEQNCSMKLGTWTYDGSVVAINPESDQPDLSNFMESGEWVIKEARGWKHWVFYSCCPTTPYLDITYHFVMQRL

Approximately 25 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of 50 mM Tris-HCL, 300 mM NaCL, 200 mM arginine, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.