CIRBP 蛋白, Mouse (His)

CIRBP protein, a key player in cellular response to genotoxic stress, stabilizes survival-associated transcripts. It aids stress granule assembly, suppresses cell proliferation in the cold, and regulates translation. CIRBP interacts with EIF4G1, associates with ribosomes, and targets the 3'-UTRs of stress-responsive transcripts like RPA2 and TXN. It contributes to intricate molecular regulatory mechanisms. CIRBP Protein, Mouse (His) is the recombinant mouse-derived CIRBP protein, expressed by E. coli , with N-6*His labeled tag. The total length of CIRBP Protein, Mouse (His) is 172 a.a., with molecular weight of ~24 kDa.

CIRBP 是一种冷诱导 mRNA 结合蛋白，是细胞对基因毒性应激反应的关键组成部分，通过稳定与细胞存活相关的转录本发挥保护功能。这种多面蛋白在过度表达时在应激颗粒 (SG) 组装中发挥关键作用，并且对于寒冷诱导的细胞增殖抑制至关重要。其多功能性包括充当翻译抑制子和激活子，对 RPA2 和 TXN 等应激反应转录本的 3'-非翻译区 (3'-UTR) 具有特定的亲和力。此外，CIRBP 与 EIF4G1 形成相互作用并与核糖体结合，强调了它在分子水平上参与复杂的调节机制。

Mouse

E. coli

N-6*His

P60824 (M1-E172)

12696  [NCBI]

MASDEGKLFVGGLSFDTNEQALEQVFSKYGQISEVVVVKDRETQRSRGFGFVTFENIDDAKDAMMAMNGKSVDGRQIRVDQAGKSSDNRSRGYRGGSAGGRGFFRGGRSRGRGFSRGGGDRGYGGGRFESRSGGYGGSRDYYASRSQGGSYGYRSSGGSYRDSYDSYATHNE

Approximately 24 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.