CKAP1/TBCB 蛋白, Human (His)

CKAP1/TBCB protein plays a pivotal role in the tubulin folding pathway, binding to alpha-tubulin folding intermediates after their chaperonin interaction, crucial for tubulin heterodimer transition. It regulates heterodimer dissociation, potentially acting as a negative regulator of axonal growth. The supercomplex, formed with cofactors A to E, captures and stabilizes tubulin, releasing committed polypeptides to the native state. CKAP1/TBCB's interactions with GAN and DCTN1 extend its functional roles in cellular processes. CKAP1/TBCB Protein, Human (His) is the recombinant human-derived CKAP1/TBCB protein, expressed by E. coli, with N-His labeled tag. The total length of CKAP1/TBCB Protein, Human (His) is 244 a.a., with molecular weight of 33-37 kDa.

CKAP1/TBCB 蛋白在微管蛋白折叠途径中表现出多方面的作用，在与胞质伴侣蛋白相互作用后与 α-微管蛋白折叠中间体结合，这是从新合成的微管蛋白到正确折叠的异二聚体转变的关键步骤。它在调节微管蛋白异二聚体解离中起着至关重要的作用，并且可能作为轴突生长的负调节因子。由辅助因子 A 至 E 组成的超级复合物的形成是其机制不可或缺的一部分。辅因子 A 和 D 通过捕获和稳定微管蛋白的准天然构象发挥作用，而辅因子 E 则与辅因子 D-微管蛋白复合物结合。随后与辅因子 C 的相互作用导致微管蛋白多肽释放至天然状态。此外，辅因子 B 和 E 可以形成与 α-微管蛋白结合的异二聚体，增强其解离微管蛋白异二聚体的能力。CKAP1/TBCB 还与 GAN 和 DCTN1 相互作用，进一步扩展其在细胞过程中的功能关联。

Human

E. coli

N-His

Q99426 (M1-I244)

1155  [NCBI]

33-37 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.