Langerin/CD207 蛋白, Human (HEK293, His)

CLC4K protein, a calcium-dependent lectin, promotes antigen uptake. CLC4K is able to bind to sulfated as well as mannosylated glycans, keratan sulfate (KS), and β-glucan. Langerin/CD207 Protein, Human (HEK293, His) is the recombinant human-derived Langerin/CD207 protein, expressed by HEK293 , with N-6*His labeled tag.

CLC4K 蛋白，是 CD207 编码的钙依赖性凝集素，属于 C 型凝集素结构域家族。CLC4K 具有甘露糖结合特异性，参与抗原呈递给 T 细胞的过程。CLC4K 是原代朗格汉斯细胞上念珠菌属、酵母菌属和糠秕马拉色菌的主要受体。朗格汉斯细胞是位于表皮和粘膜上皮内的特殊抗原呈递细胞。与朗格汉斯细胞接触后，病原体被 C 型凝集素朗格蛋白捕获，并内化到结构独特的囊泡中，称为伯贝克颗粒（BG）。CLC4K 诱导伯贝克颗粒的形成，可预防人类免疫缺陷病毒 1 (HIV-1) 感染。伯贝克颗粒生物发生过程中存在膜拉链的分子机制，而 CLC4K 是膜叠加和拉链的有效调节剂。CLC4K 与包膜糖蛋白上存在的高甘露糖结构结合，随后将病毒靶向伯贝克颗粒，导致其快速降解。朗格汉斯细胞组织细胞增多症 (LCH) 是一种以髓系前体细胞克隆扩张为特征的疾病，这些前体细胞在病变中分化为 CD1a1/CD207 细胞。所以检测是否有 CD1a、CD207 (Langerin) 和 S100 表达的克隆性肿瘤增殖，可帮助诊断 LCH。

Human

HEK293

N-6*His

AAH22278.1 (Y64-P328)

50489  [NCBI]

YPRFMGTISDVKTNVQLLKGRVDNISTLDSEIKKNSDGMEAAGVQIQMVNESLGYVRSQFLKLKTSVEKANAQIQILTRSWEEVSTLNAQIPELKSDLEKASALNTKIRALQGSLENMSKLLKRQNDILQVVSQGWKYFKGNFYYFSLIPKTWYSAEQFCVSRNSHLTSVTSESEQEFLYKTAGGLIYWIGLTKAGMEGDWSWVDDTPFNKVQSVRFWIPGEPNNAGNNEHCGNIKAPSLQAWNDAPCDKTFLFICKRPYVPSEP

30-40 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.