1. 重组蛋白
  2. Receptor Proteins
  3. CMG-2/ANTXR2 蛋白, Human (HEK293, Fc)

CMG-2/ANTXR2 蛋白, Human (HEK293, Fc)

目录号: HY-P76151
产品使用指南

CMG-2/ANTXR2 对于细胞与层粘连蛋白和细胞外基质的相互作用至关重要。作为炭疽芽孢杆菌保护性抗原 (PA) 的受体,它在 PA 结合后发生七聚化。该复合物通过网格蛋白依赖性途径内化,并在内体膜(pH < 7)中重新排列形成孔,促进炭疽毒素的其他成分进入细胞质。CMG-2/ANTXR2 蛋白, Human (HEK293, Fc) 是重组的 CMG-2/ANTXR2 蛋白,由 HEK293 表达,带有 C-hFc 标签。

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描述

CMG-2/ANTXR2 is essential for cellular interactions with laminin and the extracellular matrix. As a receptor for the protective antigen (PA) of B. anthracis, it undergoes heptamerization upon PA binding. The complex is internalized through a clathrin-dependent pathway, and in the endosomal membrane (pH < 7), it rearranges to form a pore, facilitating the entry of other components of anthrax toxin into the cytoplasm. CMG-2/ANTXR2 Protein, Human (HEK293, Fc) is the recombinant human-derived CMG-2/ANTXR2 protein, expressed by HEK293 , with C-hFc labeled tag.

研究背景

CMG-2/ANTXR2 蛋白对于细胞与层粘连蛋白和细胞外基质的相互作用至关重要。在微生物感染的情况下,它充当炭疽杆菌保护性抗原(PA)的受体。PA 结合触发受体-PA 复合物的七聚化,并且该复合物易位至富含鞘糖脂的脂筏,并通过网格蛋白依赖性途径进行内化。在内体膜内,在酸性条件下(pH 低于 7),复合物发生重排,形成孔,促进炭疽毒素的其他成分逃逸到细胞质中。这一过程凸显了 CMG-2/ANTXR2 在细胞对炭疽杆菌感染的反应以及随后炭疽毒素成分的内化和易位中的关键作用。

种属

Human

表达系统

HEK293

标签

C-hFc

蛋白编号

P58335-4 (Q34-N317)

基因 ID
蛋白结构
N-term
CMG-2 (Q34-N317)
Accession # P58335-4
hFc
C-term
同用名
Anthrax toxin receptor 2; Capillary morphogenesis gene 2 protein; CMG-2; ANTXR2
分子量

Approximately 57.4 kDa.

纯度

Greater than 85% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CMG-2/ANTXR2 Protein, Human (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CMG-2/ANTXR2 Protein, Human (HEK293, Fc)
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HY-P76151
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