CNTF 蛋白, Rat

CNTF is a pluripotent neurotrophic factor, belonging to the IL-6 cytokine family^[1]. CNTF could protect retinal cone and rod photoreceptors^[2]. CNTF has neuroprotective effects on a variety of central and also peripheral nervous system neurons^[3]. CNTF Protein, Rat is produced by E. coli with tag freeg.

睫状神经营养因子 (CNTF) 属于 IL-6 细胞因子家族。IL-6、IL-11 和 CNTF 与细胞因子反式信号传导相关。CNTF 与 IL-6 受体亚基 α (IL-6Rα) 表现出低亲和力相互作用，导致 IL-6Rβ/gp130/LIFR 信号受体复合物的形成和激活^[1]。CNTF 也是神经视网膜和感光细胞间基质中的一种细胞外信号蛋白，与 RPE、Muller 和感光细胞的膜相关^[2]。CNTF 对多种中枢和周围神经系统神经元具有神经保护作用。因为它在体外条件下促进少突胶质细胞前体细胞向少突胶质细胞的分化和成熟，从而改善髓鞘再生。重要的是，它还能提高成熟少突胶质细胞的存活率^[3]。人CNTF蛋白序列与小鼠和大鼠的相似性分别为81.82%和84.0%。

CNTF (大鼠; 1 ng/mL; 3-21 d) 影响新生大鼠分离交感神经细胞培养物中胆碱乙酰转移酶 (CHAT) 和酪氨酸羟化酶 (TH) 的水平^[4]。

The ED₅₀ is <30 ng/mL as measured by its ability to induce alkaline phosphatase production by TF-1 cells.

Rat

E. coli

Tag Free

P20294 (A2-M200)

25707  [NCBI]

AFAEQTPLTLHRRDLCSRSIWLARKIRSDLTALMESYVKHQGLNKNINLDSVDGVPVASTDRWSEMTEAERLQENLQAYRTFQGMLTKLLEDQRVHFTPTEGDFHQAIHTLMLQVSAFAYQLEELMVLLEQKIPENEADGMPATVGDGGLFEKKLWGLKVLQELSQWTVRSIHDLRVISSHQMGISALESHYGAKDKQM

Approximately 22.9 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against 50 mM Tris, pH 8.0.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Jones SA, et al. Recent insights into targeting the IL-6 cytokine family in inflammatory diseases and cancer. Nat Rev Immunol. 2018 Dec;18(12):773-789.  [Content Brief]

  [2]. Li S, et al. Ciliary neurotrophic factor (CNTF) protects retinal cone and rod photoreceptors by suppressing excessive formation of the visual pigments. J Biol Chem. 2018 Sep 28;293(39):15256-15268.  [Content Brief]

  [3]. Abbaszadeh HA, et al. Human ciliary neurotrophic factor-overexpressing stable bone marrow stromal cells in the treatment of a rat model of traumatic spinal cord injury. Cytotherapy. 2015 Jul;17(7):912-21.  [Content Brief]

  [4]. Saadat S, et al. Ciliary neurotrophic factor induces cholinergic differentiation of rat sympathetic neurons in culture[J]. The Journal of cell biology, 1989, 108(5): 1807-1816.