Coagulation Factor II/F2 蛋白, Mouse (HEK293, His)

Coagulation Factor II/F2, also known as thrombin, functions as a pivotal serine protease. Its enzymatic action involves cleaving bonds after Arg and Lys residues, facilitating the conversion of fibrinogen to fibrin. Thrombin additionally activates key factors like V, VII, VIII, XIII, and, in conjunction with thrombomodulin, protein C. Beyond blood homeostasis, thrombin plays a role in inflammation and the complex dynamics of wound healing. Coagulation Factor II/F2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Coagulation Factor II/F2 protein, expressed by HEK293 , with C-His labeled tag. The total length of Coagulation Factor II/F2 Protein, Mouse (HEK293, His) is 594 a.a., with molecular weight of ~85 kDa.

凝血因子 II/F2 蛋白，俗称凝血酶，在各种生理过程中发挥着关键作用。作为丝氨酸蛋白酶，凝血酶裂解精氨酸和赖氨酸残基后的键，导致纤维蛋白原转化为纤维蛋白。此外，凝血酶可激活几个关键因子，包括 V、VII、VIII、XIII，以及与血栓调节蛋白结合使用时的蛋白 C。除了在血液稳态中的基本作用外，凝血酶还有助于炎症和伤口愈合的复杂过程。

Measured by its ability to cleave a peptide substrate, BOC-Val-Pro-Arg-AMC. Read at excitation and emission wavelengths of 380 nm and 460 nm (top read). The specific activity is 25888.119 pmoL/min/µg, as measured under the described conditions.

Mouse

HEK293

C-His

Q3TJ94 (Q25-G618)

14061  [NCBI]

QHVFLAPQQALSLLQRVRRANSGFLEELRKGNLERECVEEQCSYEEAFEALESPQDTDVFWAKYTVCDSVRKPRETFMDCLEGRCAMDLGVNYLGTVNVTHTGIQCQLWRSRYPHKPEINSTTHPGADLKENFCRNPDSSTTGPWCYTTDPTVRREECSVPVCGQEGRTTVVMTPRSGGSKDNLSPPLGQCLTERGRLYQGNLAVTTLGSPCLPWNSLPAKTLSKYQDFDPEVKLVENFCRNPDWDEEGAWCYVAGQPGDFEYCNLNYCEEAVGEENYDVDESIAGRTTDAEFHTFFNEKTFGLGEADCGLRPLFEKKSLKDTTEKELLDSYIDGRIVEGWDAEKGIAPWQVMLFRKSPQELLCGASLISDRWVLTAAHCILYPPWDKNFTENDLLVRIGKHSRTRYERNVEKISMLEKIYVHPRYNWRENLDRDIALLKLKKPVPFSDYIHPVCLPDKQTVTSLLRAGYKGRVTGWGNLRETWTTNINEIQPSVLQVVNLPIVERPVCKASTRIRITDNMFCAGFKVNDTKRGDACEGDSGGPFVMKSPFNNRWYQMGIVSWGEGCDRKGKYGFYTHVFRLKRWIQKVIDQFG

Approximately 85 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.