Coagulation Factor IX/F9 蛋白, Mouse (HEK293, His)

Coagulation Factor IX/F9 Protein, a vital vitamin K-dependent plasma protein, is pivotal in the intrinsic blood coagulation pathway. Its role includes converting factor X into its active form, a process facilitated by Ca(2+) ions, phospholipids, and factor VIIIa. Coagulation Factor IX/F9 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Coagulation Factor IX/F9 protein, expressed by HEK293 , with C-His labeled tag. The total length of Coagulation Factor IX/F9 Protein, Mouse (HEK293, His) is 471 a.a., with molecular weight of 60-95 kDa.

凝血因子 IX/F9 蛋白是一种维生素 K 依赖性血浆蛋白，在凝血的内在途径中起着至关重要的作用。其功能涉及将因子 X 转化为其活性形式，并且该过程在 Ca(2+) 离子、磷脂和因子 VIIIa 存在的情况下得到促进。

Measured by its ability to cleave 10μM fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is 227.339 pmol/min/μg, as measured under the described conditions.

Mouse

HEK293

C-10*His

P16294 (A29-T471)

14071  [NCBI]

AVFLDRENATKILTRPKRYNSGKLEEFVRGNLERECIEERCSFEEAREVFENTEKTTEFWKQYVDGDQCESNPCLNGGICKDDISSYECWCQVGFEGRNCELDATCNIKNGRCKQFCKNSPDNKVICSCTEGYQLAEDQKSCEPTVPFPCGRASISYSSKKITRAETVFSNMDYENSTEAVFIQDDITDGAILNNVTESSESLNDFTRVVGGENAKPGQIPWQVILNGEIEAFCGGAIINEKWIVTAAHCLKPGDKIEVVAGEYNIDKKEDTEQRRNVIRTIPHHQYNATINKYSHDIALLELDKPLILNSYVTPICVANREYTNIFLKFGSGYVSGWGKVFNKGRQASILQYLRVPLVDRATCLRSTTFTIYNNMFCAGYREGGKDSCEGDSGGPHVTEVEGTSFLTGIISWGEECAMKGKYGIYTKVSRYVNWIKEKTKLT

Approximately 60-95 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.