COASY 蛋白, Human (His)

COASY protein is a bifunctional enzyme that plays a key role in catalyzing the fourth and fifth steps of the CoA biosynthetic pathway. The coaD domain promotes the phosphopantothenate adenylyltransferase activity of the fourth reaction, and the coaE domain drives the dephosphoCoA kinase activity of the fifth reaction. COASY Protein, Human (His) is the recombinant human-derived COASY protein, expressed by E. coli , with N-His labeled tag.

COASY 蛋白作为双功能酶发挥作用，在催化 CoA 生物合成途径的第四和第五连续步骤中发挥关键作用。由 coaD 结构域编码的磷酸泛硫氨酸腺苷酰转移酶活性促进第四个反应，而由 coaE 结构域编码的去磷酸 CoA 激酶活性驱动该途径中的第五个反应。这种酶是 CoA 生物合成调节不可或缺的一部分，可能作为控制整个过程的关键点，确保 CoA 的正确和有效合成，CoA 是参与各种细胞代谢途径的重要辅助因子。

Measured in a cell proliferation assay using MDA-MB-231 cells. The ED₅₀ for this effect is typically 15.42 ng/mL, corresponding to a specific activity is 64850.843 units/mg.

• 
  Measured in a cell proliferation assay using MDA-MB-231 cells. The ED₅₀ for this effect is typically 15.42 ng/mL, corresponding to a specific activity is 64850.843 units/mg.

Human

E. coli

N-6*His

Q13057-1 (M296-D564)

80347  [NCBI]

MAINRFRLENDLEELALYQIQLLKDLRHTENEEDKVSSSSFRQRMLGNLLRPPYERPELPTCLYVIGLTGISGSGKSSIAQRLKGLGAFVIDSDHLGHRAYAPGGPAYQPVVEAFGTDILHKDGIINRKVLGSRVFGNKKQLKILTDIMWPIIAKLAREEMDRAVAEGKRVCVIDAAVLLEAGWQNLVHEVWTAVIPETEAVRRIVERDGLSEAAAQSRLQSQMSGQQLVEQSHVVLSTLWEPHITQRQVEKAWALLQKRIPKTHQALD

Approximately 32 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 300 mM NaCl, pH 6.6, 8% trehalose, 8% mannitol and 0.02% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.