Complement C5/C5a 蛋白, Human

Complement C5/C5a Protein, Human is a recombinant human complement C5a. C5a is a 74-amino acid glycoprotein generated on activation of the C system. Complement C5 is cleaved by proteolysis in the terminal phase of complement activation generating the pro-inflammatory C5a and membrane attack complex nucleator C5b. C5a is an inflammatory mediator generated by complement activation that positively regulates various arms of immune defense, including Toll-like receptor 4 (TLR4) signaling^[1][2].

补体成分 5a (C5a) 是一种由 74 个氨基酸组成的糖蛋白，是一种重要的促炎介质，在补体级联激活时，可通过酶促作用从其前体 C5 上裂解下来。C5a 很快被羧肽酶代谢，形成效力较低的 C5a desArg。C5a 和 C5a desArg 与其受体（C5aR 和 C5L2）相互作用，从而产生许多对免疫反应至关重要的效应。C5a 在整个人体中具有广泛的生物效应，因为 C5a 受体在整个人体器官中广泛表达，使得 C5a 和 C5a desArg 能够引发广泛的生物效应^[2]。

1.Immobilized C5a at 2 μg/mL (100 μL/well) can bind anti-C5a. The ED₅₀ is 41.72 ng/mL, corresponding to a specific activity is 2.39×10⁴ units/mg.
2.Measured by its ability to induce N-acetyl-beta-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. The ED₅₀ for this effect is 3.332-4.647 ng/mL, corresponding to a specific activity is 2.151-3.001×10⁵ units/mg.

• 
  Immobilized C5a at 2 μg/mL (100 μL/well) can bind anti-C5a,  The ED₅₀ for this effect is 41.72 ng/mL，corresponding to a specific activity is 2.39×10⁴ units/mg.

Human

E. coli

Tag Free

P01031 (T678-R751)

727  [NCBI]

TLQKKIEEIAAKYKHSVVKKCCYDGACVNNDETCEQRAARISLGPRCIKAFTECCVVASQLRANISHKDMQLGR

Approximately 10 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. An G, et al. Role of C5a-C5aR axis in the development of atherosclerosis. Sci China Life Sci. 2014;57(8):790-794.  [Content Brief]

  [2]. Haggadone MD, et al. Bidirectional Crosstalk between C5a Receptors and the NLRP3 Inflammasome in Macrophages and Monocytes. Mediators Inflamm. 2016;2016:1340156.  [Content Brief]