CRACC/SLAMF7 蛋白, Human (Biotinylated, HEK293, His-Avi)

The FITC-labeled CRACC/SLAMF7 protein, a self-ligand receptor in the SLAM family, intricately regulates immune cell activation and differentiation through finely tuned interactions. Isoform 1 mediates NK cell activation via a SH2D1A-independent ERK-mediated pathway, positively regulating NK cell functions dependent on phosphorylated SH2D1B. It inhibits NK cell function in the absence of SH2D1B and acts as an inhibitory factor in T-cells. In LPS-activated monocytes, it negatively regulates pro-inflammatory cytokine production. However, isoform 3 shows no NK cell activation, suggesting isoform-specific immune modulation. CRACC/SLAMF7 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CRACC/SLAMF7 protein, expressed by HEK293 , with C-Avi, C-6*His labeled tag. The total length of CRACC/SLAMF7 Protein, Human (Biotinylated, HEK293, His-Avi) is 204 a.a., with molecular weight of 35-55 kDa.

FITC 标记的 CRACC/SLAMF7 蛋白作为信号淋巴细胞激活分子 (SLAM) 家族中的自配体受体，参与同型或异型细胞间相互作用，调节各种免疫细胞的激活和分化，错综复杂地有助于先天性和适应性免疫反应的调节和相互联系。该蛋白质的活性可通过小细胞质接头蛋白（包括 SH2D1A/SAP 和/或 SH2D1B/EAT-2）的存在或不存在进行微调。具体而言，FITC 标记的 CRACC/SLAMF7 亚型 1 通过不依赖于 SH2D1A 的细胞外信号调节 ERK 介导途径介导 NK 细胞活化，以依赖于磷酸化 SH2D1B 的机制正向调节 NK 细胞功能。下行信令涉及PLCG1、PLCG2和PI3K。此外，NK 细胞之间的同型相互作用可能有助于激活，但在缺乏 SH2D1B 的情况下，FITC 标记的 CRACC/SLAMF7 会抑制 NK 细胞功能。该蛋白还充当 T 细胞的抑制因子，并可能在淋巴细胞粘附中发挥作用。在脂多糖激活的单核细胞中，它负向调节促炎细胞因子的产生。然而，FITC 标记的 CRACC/SLAMF7 的异构体 3 不介导任何 NK 细胞激活，表明免疫调节中异构体特异性的功能差异。

Human

HEK293

C-Avi;C-6*His

Q9NQ25-1 (S23-M226)

57823  [NCBI]

SGPVKELVGSVGGAVTFPLKSKVKQVDSIVWTFNTTPLVTIQPEGGTIIVTQNRNRERVDFPDGGYSLKLSKLKKNDSGIYYVGIYSSSLQQPSTQEYVLHVYEHLSKPKVTMGLQSNKNGTCVTNLTCCMEHGEEDVIYTWKALGQAANESHNGSILPISWRWGESDMTFICVARNPVSRNFSSPILARKLCEGAADDPDSSM

35-55 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.