CRIPT 蛋白, Human (His)

CRIPT, a pivotal member of the minor spliceosome, plays a crucial role in U12-type intron splicing, contributing to RNA splicing intricacies. Beyond its splicing function, CRIPT anchors DLG4 within excitatory synapses, interacts with RNF113A, SF3B1/SF3b155, SF3B2/SF3b145, and PHF5A/SF3b14b in the minor spliceosome complex, and strongly associates with the PDZ3 domain of DLG4 family members, highlighting its synaptic involvement. Additionally, CRIPT interacts with microtubules, indicating a role in cytoskeletal dynamics, showcasing its multifaceted contributions. CRIPT Protein, Human (His) is the recombinant human-derived CRIPT protein, expressed by E. coli, with N-His labeled tag. The total length of CRIPT Protein, Human (His) is 101 a.a., with molecular weight of ~14 kDa.

CRIPT 是小剪接体的重要参与者，在前体 mRNA 中 U12 型内含子的剪接中发挥着重要作用，有助于 RNA 剪接的复杂过程。除了在剪接中的作用外，CRIPT 还参与 DLG4 在兴奋性突触内的细胞骨架锚定。作为次要剪接体复合物的组成部分，CRIPT 与 RNF113A、SF3B1/SF3b155、SF3B2/SF3b145 和 PHF5A/SF3b14b 等关键伙伴相互作用。它还表现出与 DLG4 家族成员的 PDZ3 结构域的强烈相互作用，强调了其在突触功能中的作用。此外，CRIPT 与微管相关，表明它参与细胞骨架动力学。CRIPT 与各种分子伙伴的复杂相互作用强调了它对剪接和突触组织的多方面贡献。

Human

E. coli

N-His

Q9P021 (M1-V101)

9419  [NCBI]

MVCEKCEKKLGTVITPDTWKDGARNTTESGGRKLNENKALTSKKARFDPYGKNKFSTCRICKSSVHQPGSHYCQGCAYKKGICAMCGKKVLDTKNYKQTSV

Approximately 14 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 300 mM NaCl, 5% trehalose, 5% mannitol and 0.01% Tween80, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.