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  3. CRKL 蛋白, Human (His, Strep)

CRKL 蛋白, Human (His, Strep)

目录号: HY-P702049
产品使用指南

CRKL 蛋白介导细胞内信号转导,与酪氨酸磷酸化 EPOR 和 INPP5D/SHIP1 相互作用,并在促红细胞生成素受体和磷酸肌醇信号传导中发挥作用。它还与 DOCK2 和 DOCK5 结合,表明鸟嘌呤核苷酸交换因子受到调节。CRKL 蛋白, Human (His, Strep) 是重组的 CRKL 蛋白,由 E. coli 表达,带有 N-Strep, N-6*His 标签。CRKL 蛋白, Human (His, Strep) 全长 303 个氨基酸。

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描述

CRKL protein mediates intracellular signal transduction, interacts with tyrosine-phosphorylated EPOR and INPP5D/SHIP1, and plays a role in erythropoietin receptor and phosphoinositide signaling. It also binds to DOCK2 and DOCK5, suggesting regulation of guanine nucleotide exchange factors. CRKL Protein, Human (His, Strep) is the recombinant human-derived CRKL protein, expressed by E. coli , with N-Strep, N-6*His labeled tag. The total length of CRKL Protein, Human (His, Strep) is 303 a.a., .

研究背景

CRKL 蛋白参与介导细胞内信号的转导,表明其在细胞信号传导途径中的作用。它与酪氨酸磷酸化的 EPOR 和 INPP5D/SHIP1 相互作用,表明它参与与促红细胞生成素受体信号传导和磷酸肌醇信号传导相关的途径。此外,CRKL 通过其第一个 SH3 结构域与 DOCK2 和 DOCK5 相互作用,突出了其在调节鸟嘌呤核苷酸交换因子中的潜在作用。该蛋白进一步与磷酸化的 CBLB 和 IRS4 结合,表明它参与了与细胞增殖和胰岛素受体信号传导相关的途径。此外,CRKL 与 BCAR1/CAS 和 NEDD9/HEF1 相互作用,表明其参与与细胞粘附和迁移相关的信号级联反应。这些不同的相互作用强调了 CRKL 在细胞内传递信号方面的多功能性。

种属

Human

表达系统

E. coli

标签

N-Strep;N-6*His

蛋白编号

P46109 (M1-E303)

基因 ID

1399

蛋白结构
N-term
Strep-6*His
CRKL (M1-E303)
Accession # P46109
C-term
同用名
CRKL; Crk-like protein
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CRKL Protein, Human (His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CRKL Protein, Human (His, Strep)
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HY-P702049
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