CSF1R 蛋白, Rat (HEK293, C-His)

CSF1R Protein is a cell surface receptor with high a affinity for a variety of polypeptide growth factors, cytokines, and hormones. CSF1R Protein participate in PLCG2/PKA/UCP2 signaling pathway to reduce oxidative stress and neuronal apoptosis in rat models with neonatal HIE. The blocking of the CSF1R signal is associated with tumorigenesis. CSF1R Protein, Rat (HEK293, C-His) is the recombinant rat-derived CSF1R, expressed by HEK293 , with C-10*His labeled tag. The total length of CSF1R Protein, Rat (HEK293, C-His) is 491 a.a.,

Receptor protein-tyrosine kinase 是一种对多种多肽类生长因子、细胞因子，及激素具有高亲和性细胞表面受体。与人 CSF1R (集落刺激因子 1 受体) 同源。CSF1R 具有巨噬细胞集落刺激因子受体活性、蛋白同型二聚化活性和和蛋白磷酸酶结合活性。CSF1R 参与破骨细胞分化的正向调节以及骨吸收的调节。CSF1R 参与 PLCG2/PKA/UCP2 信号通路减轻新生儿 HIE 大鼠模型中的氧化应激和神经元凋亡。CSF1R 信号的阻断可抑制肿瘤浸润性髓样细胞，提高前列腺癌放疗疗效。在 INS-1E beta-cells中，通过 CSF1R/IRR 受体构建激活 Erk1/2磷酸化。

Measured by its ability to inhibit the M-CSF-induced proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is 0.2793 µg/mL in the presence of 10 ng/mL mouse M-CSF, corresponding to a specific activity is 3.58×10³ units/mg.

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  Measured by its ability to inhibit the M-CSF-induced proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is 0.2793 µg/mL in the presence of 10 ng/mL mouse M-CSF, corresponding to a specific activity is 3.58×10³ units/mg.

Rat

HEK293

C-10*His

D4ACA7 (A64-E554)

307403

APVIEPSGPELVVEPGATVTLRCVSNGSVEWDGPISPYWTLDSESPGSILITKNATFKNTGTYRCTELEDPMRGSTAIHLYVKDPVRPWNLLAQEVTVFEGQEAVLPCLITDPTLKDSVSLVREWGRPVSRKTVYSFLPWRGFIIHKAKFLDSHTYMCKAVVNARESTSIGIRLKVNRAHPGPPHIILEPTKLVRIRGEAAQIVCSATHSEVEFNVILKRGDTKLEIPINSDFQDNAYKKVLTLNLNAVDFQDAGIYSCVANNAAGSNTATMNFQVVESAYLNLTSEQSLLQEVSVGENLDLTVIADAYPGLQRYNWTYLGPFFEDPHNLEFRTQWTTYSYSFKLHLNRVKPLEAGRYSLMAQNKAGWNNLTFELTLRYPPEVSVTWIPVNGSDVLLCDVSGYPQPNVTWMECRGHTDRCDEAQASQVWDDTQPEVLSQKPFHRVILQSQLPIGTLKHNMTYVCRAHNNVGNSSQFFRAISLGQSKQLPDE

Approximately 75-100 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.