CSK 蛋白, Human (sf9, GST)

CSK is a non-receptor tyrosine protein kinase that critically regulates cell growth, differentiation, migration and immune responses by phosphorylating tyrosine residues in the C-terminal tail of Src family kinases (SFK). This induces an inactive conformation through intramolecular interactions with the SH2 domain of SFK. CSK Protein, Human (sf9, GST) is the recombinant human-derived CSK protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of CSK Protein, Human (sf9, GST) is 450 a.a., with molecular weight of ~66 kDa.

CSK 是一种非受体酪氨酸蛋白激酶，在调节细胞生长、分化、迁移和免疫反应中发挥着至关重要的作用。它磷酸化 Src 家族激酶 (SFK) C 末端尾部的酪氨酸残基，包括 LCK、SRC、HCK、FYN、LYN、CSK 或 YES1。这种磷酸化会触发 SFK 的磷酸酪氨酸尾部和 SH2 结构域之间的分子内相互作用，从而诱导失活构象。为了发挥其对 SFK 的抑制功能，CSK 通过与紧邻膜的跨膜蛋白或衔接蛋白相互作用而被招募到质膜上。CSK 通过磷酸化包括 FYN 或 LCK 在内的几种正效应子并将其维持在非活性状态，有效抑制来自各种表面受体（例如 T 细胞受体 (TCR) 和 B 细胞受体 (BCR)）的信号传导。

Human

Sf9 insect cells

N-GST

P41240 (M1-L450)

1445  [NCBI]

MSAIQAAWPSGTECIAKYNFHGTAEQDLPFCKGDVLTIVAVTKDPNWYKAKNKVGREGIIPANYVQKREGVKAGTKLSLMPWFHGKITREQAERLLYPPETGLFLVRESTNYPGDYTLCVSCDGKVEHYRIMYHASKLSIDEEVYFENLMQLVEHYTSDADGLCTRLIKPKVMEGTVAAQDEFYRSGWALNMKELKLLQTIGKGEFGDVMLGDYRGNKVAVKCIKNDATAQAFLAEASVMTQLRHSNLVQLLGVIVEEKGGLYIVTEYMAKGSLVDYLRSRGRSVLGGDCLLKFSLDVCEAMEYLEGNNFVHRDLAARNVLVSEDNVAKVSDFGLTKEASSTQDTGKLPVKWTAPEALREKKFSTKSDVWSFGILLWEIYSFGRVPYPRIPLKDVVPRVEKGYKMDAPDGCPPAVYEVMKNCWHLDAAMRPSFLQLREQLEHIKTHELHL

Approximately 66 kDa

Greater than 90% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.