CUL5 蛋白, Human (Sf9)

The CUL5 protein is a core component of the SCF-like ECS E3 ubiquitin-protein ligase complex. CUL5 Protein, Human (Sf9) is the recombinant human-derived CUL5 protein, expressed by Sf9 insect cells , with tag free. The total length of CUL5 Protein, Human (Sf9) is 779 a.a., .

CUL5 蛋白是多个 SCF 样 ECS（Elongin-Cullin 2/5-SOCS-box 蛋白）E3 泛素-蛋白连接酶复合物的核心成分，协调靶蛋白的泛素化和随后的蛋白酶体降解。作为支架蛋白，CUL5 可能通过精确定位底物和泛素结合酶来发挥催化作用。这些 E3 连接酶复合物的功能特异性依赖于不同的底物识别组件。ECS(SOCS1) 参与指导 JAK2 的泛素化，而 ECS(KLHDC1) 复合体是 DesCEND 途径的一部分，促进由于 UGA/Sec 解码失败而导致的截短 SELENOS 硒蛋白的泛素化和降解。此外，CUL5 作为多亚基复合物的一部分，多聚泛素化单泛素化 POLR2A，并可能形成细胞表面加压素受体。在微生物感染的情况下，CUL5 似乎在腺病毒 E1B-55 kDa 蛋白刺激的 p53/TP53 蛋白酶体降解中发挥作用。

Human

Sf9 insect cells

Tag Free

Q93034 (A2-A780)

/

Approximately 91 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH7.5, 200 mM NaCl, 20% glycerol.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.