CXCL16 蛋白, Human (HEK293, His)

CXCL16, also known as SR-PSOX (scavenger receptor that binds phosphatidylserine and oxidized lipoprotein), is one member of the ELR-negative CXC chemokine subfamily. CXCL16 is a multifunctional protein involved in various inflammatory diseases, atherosclerosis, and cancer. CXCL16 can be observed in many cell types^[1][2]. CXCL16 Protein, Human (HEK293, His) is produced in HEK293 cells with a C-Terminal His-tag. It consists of 176 amino acids (N49-T224).

CXCL16 是一种膜结合趋化因子。CXCL16以可溶性或跨膜形式表达，可在多种细胞类型中观察到，包括炎症细胞（如巨噬细胞、中性粒细胞、树突状细胞和单核细胞）和非炎症细胞（如肺上皮细胞和肾细胞）。CXCL16在天然免疫屏障和自身免疫性疾病的发生发展中发挥着重要作用^[1][2]。
CXCL16 蛋白在小鼠、大鼠和狗 CXCL16 蛋白之间具有较低的同源性。
CXCL16 主要表达于抗原呈递细胞 (APC) 表面，由趋化因子结构域（约 89 个氨基酸）组成，是一种粘蛋白型茎（约 110 个氨基酸）、单次跨膜结构域（约 20 个氨基酸）和胞质尾（约 27 个氨基酸）。CXCL16 是 CXCR6 受体的唯一配体。可溶性CXCL16诱导CXCR6+细胞（包括Th1细胞、NK细胞和活化的 CD8+ T 细胞）的迁移、M2巨噬细胞浸润、APC 和 CD8+ T 细胞之间的相互作用、细胞免疫反应和炎症反应以及发育胸腺细胞。膜结合的 CXCL16 可以促进 CXCR6+ 细胞的粘附。CXCL16 特异性结合氧化低密度脂蛋白 (OxLDL)，导致其内化和降解。CXCL16 可能在动脉粥样硬化病变的形成中发挥重要作用。巨噬细胞和树突状细胞上的 CXCL16 介导大肠杆菌和金黄色葡萄球菌等细菌的粘附和吞噬作用，而细菌识别则由 CXCL16 的趋化因子结构域介导^[1][2]。
CXCL16 不仅是一种趋化因子，也是一种多功能蛋白。CXCL16 和 CXCR6 与多种炎症性疾病有关，例如肾小球肾炎、肺部疾病、动脉粥样硬化、冠状动脉疾病、类风湿性关节炎和许多与炎症相关的癌症。CXCL16 的趋化因子结构域对大肠杆菌和金黄色葡萄球菌发挥有效的抗微生物活性。CXCL16 通过吸引表达 CXCR6 的细胞（例如激活的 T 细胞和 NKT 细胞）充当先天免疫的介质。CXCL16 也是人类表皮角质形成细胞先天免疫反应的新型介质^[1][2][3]。

Recombinant human CXCL16 (1 ng/mL) induces cell migration in primary human monocytes or M2 macrophages^[3].

1.Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with mouse CXCR6 and the ED₅₀ is ≤250 ng/mL.
2.Measured by its ability to chemoattract activated Jurkat cells. The ED₅₀ for this effect is 0.856 ng/mL, corresponding to a specific activity is 1.168×10⁶ U/mg.

• 
  Measured by its ability to chemoattract activated Jurkat cells. The ED₅₀ for this effect is 0.856 ng/mL, corresponding to a specific activity is 1.168×10⁶ U/mg.

Human

HEK293

C-His

Q9H2A7 (N30-T205)/NP_071342.2 (N49-T224)

58191  [NCBI]

NEGSVTGSCYCGKRISSDSPPSVQFMNRLRKHLRAYHRCLYYTRFQLLSWSVCGGNKDPWVQELMSCLDLKECGHAYSGIVAHQKHLLPTSPPISQASEGASSDIHTPAQMLLSTLQSTQRPTLPVGSLSSDKELTRPNETTIHTAGHSLAAGPEAGENQKQPEKNAGPTARTSAT

Approximately 38-42 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Tohyama M, et al. CXCL16 is a novel mediator of the innate immunity of epidermal keratinocytes. Int Immunol. 2007 Sep;19(9):1095-102.  [Content Brief]

  [2]. Jianhui Sun, et al. A Functional Variant of CXCL16 Is Associated With Predisposition to Sepsis and MODS in Trauma Patients: Genetic Association Studies. Front Genet. 2021 Sep 3;12:720313.  [Content Brief]

  [3]. Allaoui R, et al. Cancer-associated fibroblast-secreted CXCL16 attracts monocytes to promote stroma activation in triple-negative breast cancers. Nat Commun. 2016 Oct 11;7:13050.  [Content Brief]