CXCR4 蛋白, Human (Cell-Free, His)

The CXCR4 protein functions as a receptor for the CXC chemokine CXCL12/SDF-1, triggering an increase in intracellular calcium ions and activation of MAPK1/MAPK3. It is actively involved in AKT signaling, which is critical for regulating cell migration, especially in wound healing. CXCR4 Protein, Human (Cell-Free, His) is the recombinant human-derived CXCR4 protein, expressed by E. coli Cell-free , with N-6*His labeled tag. The total length of CXCR4 Protein, Human (Cell-Free, His) is 356 a.a., the molecular weight are 44 KDa (monomer), 100 KDa (dimer), whiel dimers are generally formed.

CXCR4 蛋白作为 CXC 趋化因子 CXCL12/SDF-1 的受体，传递增加细胞内钙离子水平并增强 MAPK1/MAPK3 激活的信号。它积极参与 AKT 信号级联反应，并在调节细胞迁移中发挥至关重要的作用，特别是在伤口愈合等过程中。此外，CXCR4 作为细胞外泛素的受体，导致细胞内钙离子升高和细胞 cAMP 水平降低。它还结合细菌脂多糖 (LPS) 并介导 LPS 诱导的炎症反应，包括单核细胞分泌 TNF。除了其免疫功能外，CXCR4 在造血、心室间隔形成、胃肠道血管化和小脑发育中发挥着重要作用。在中枢神经系统中，它可能介导海马神经元的存活。此外，在微生物感染的情况下，CXCR4 与 CD4 一起充当人类免疫缺陷病毒 1 (HIV-1) X4 分离株的辅助受体，并充当某些 HIV-2 分离株的主要受体，促进病毒融合。

Human

E. coli Cell-free

N-6*His

P61073-2 (M1-S356)

7852  [NCBI]

MSIPLPLLQIYTSDNYTEEMGSGDYDSMKEPCFREENANFNKIFLPTIYSIIFLTGIVGNGLVILVMGYQKKLRSMTDKYRLHLSVADLLFVITLPFWAVDAVANWYFGNFLCKAVHVIYTVNLYSSVLILAFISLDRYLAIVHATNSQRPRKLLAEKVVYVGVWIPALLLTIPDFIFANVSEADDRYICDRFYPNDLWVVVFQFQHIMVGLILPGIVILSCYCIIISKLSHSKGHQKRKALKTTVILILAFFACWLPYYIGISIDSFILLEIIKQGCEFENTVHKWISITEALAFFHCCLNPILYAFLGAKFKTSAQHALTSVSRGSSLKILSKGKRGGHSSVSTESESSSFHSS

Observed band size: Monomer: 44 kDa Dimer: 100 kDa It is speculated that the protein forms a dimeric structure.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, 0.05% EOS-12, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.