CYBB/Nox2 蛋白, Human (His)

The CYBB/Nox2 protein is an important membrane-bound oxidase in phagocytes and is critical for superoxide production. As the terminal element of the respiratory chain, it transfers a single electron from NADPH to molecular oxygen. CYBB/Nox2 Protein, Human (His) is the recombinant human-derived CYBB/Nox2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of CYBB/Nox2 Protein, Human (His) is 288 a.a., with molecular weight of ~37.2 kDa.

CYBB/Nox2 蛋白是吞噬细胞中膜结合氧化酶的重要组成部分，在超氧化物的生成中发挥着关键作用。作为呼吸链的末端元件，它促进单电子从细胞质 NADPH 穿过质膜转移到外部的分子氧。此外，CYBB/Nox2 作为电压门控质子通道运行，协调静息吞噬细胞中的 H(+) 电流。它的参与延伸到细胞 pH 值的调节，值得注意的是，这种功能是由锌来调节的，锌是通道的阻断剂。CYBB/Nox2 的多方面活性强调了其在与吞噬细胞内氧化和质子调节相关的复杂细胞过程中的重要性。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

P04839 (E283-F570)

1536  [NCBI]

ERLVRFWRSQQKVVITKVVTHPFKTIELQMKKKGFKMEVGQYIFVKCPKVSKLEWHPFTLTSAPEEDFFSIHIRIVGDWTEGLFNACGCDKQEFQDAWKLPKIAVDGPFGTASEDVFSYEVVMLVGAGIGVTPFASILKSVWYKYCNNATNLKLKKIYFYWLCRDTHAFEWFADLLQLLESQMQERNNAGFLSYNIYLTGWDESQANHFAVHHDEEKDVITGLKQKTLYGRPNWDNEFKTIASQHPNTRIGVFLCGPEALAETLSKQSISNSESGPRGVHFIFNKENF

Approximately 37 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.